Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

HAT activity assay kit

 
ALX-850-326-KI01 100 tests 564.00 USD
Do you need bulk/larger quantities?
 
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
850-326
Figure 1: Analysis of HAT Activity in HeLa Nuclear Extract. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a microplate reader at 440nm according to the kit instructions.
Please mouse over
850-326

Product Specification

Alternative Name:Histone acetyltransferase
 
Applications:Colorimetric detection
Activity assay
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Kit/Set Contains:2x HAT Assay Buffer
HAT Substrate I
HAT Substrate II
NADH Generating Enzyme
NE (Nuclear Extract 4 mg/ml)
HAT Reconstitution Buffer
 

Product Literature References

Differential responses of the right ventricle to abnormal loading conditions in vivo: Possible pathophysiologic mechanisms : A. Azakie, et al.; J. Thorac. Cardiovasc. Surg. 145, 1335 (2013), Abstract;
CPTH6, a thiazole derivative, induces histone hypoacetylation and apoptosis in human leukemia cells: D. Trisciuoglio, et al.; Clin. Cancer Res. 18, 475 (2012), Application(s): Measurement of HAT activity in CPTH6-treated cells, Abstract;
Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells: P.A. Ruiz, et al.; J. Nutr. 137, 1208 (2007), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
HAT
By biological activity:
HAT Activity kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Brochures
Epigenetics
Epigenetics
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,