HAT activity assay kit



ALX-850-326-KI01	1 Kit	491.00 USD

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH.

Figure 1: Analysis of HAT Activity in HeLa Nuclear Extract. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a microplate reader at 440nm according to the kit instructions.

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Product Specification

Quantity:	~100 tests.

Kit/Set Contains:	2x HAT Assay Buffer HAT Substrate I HAT Substrate II NADH Generating Enzyme NE (Nuclear Extract 4 mg/ml) HAT Reconstitution Buffer

Long Term Storage:	-20°C

Product Literature References

Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells: P.A. Ruiz, et al.; J. Nutr. 137, 1208 (2007), Abstract;