Specific – antibody to Adda moiety specifically binds microcystin & nodularin toxins
Sensitive – measure as little as 0.1ng/ml toxin
Convenient – no pre-sample preparation required
Rapid – analyze 41 duplicate samples in <2.5 hours
Polyclonal antibody prepared against the Adda moiety binds to microcystins and nodularins, allowing the congener-independent determination of these toxins and many of its congeners, and does not cross-react with other non-related toxins or compounds. No pre-sample preparation required. Total time for measurement is less than 2.5 hours. Enables simultaneous measurement of multiple samples at reasonable costs.
Cross-reactivity pattern against microcystins and nodularin congeners
Please mouse over
Product Details
Sensitivity:
0.1ng/ml (range 0.15 to 5ng/ml)
Assay Time:
2 hour 30 minutes
Applications:
ELISA
Application Notes:
For the quantitative and sensitive congener-independent detection of microcystins and nodularin in water samples.
Wavelength:
450 nm
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Scientific Background:
Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
Technical Info/Product Notes:
Patented technology. U.S. Patent No. 6,967,240. Worldwide Patent PCT WO 01/18059 A2.
Adsorption behavior of polyamide microplastics as a vector of the cyanotoxin microcystin-LR in environmental freshwaters: N. Kim, et al.; J. Hazard. Mater. 446, 130683 (2023), Abstract;
Combined effects of increased water temperature and cyanobacterial compounds exert heterogeneous effects on survival and ecological processes in key freshwater species: O.A. Adekolurejo, et al.; Oecologia 200, 515 (2022), Abstract;
An ELISA-based Method for Variant-independent Detection of Total 3 Microcystins and Nodularins via Multi-immunogen Approach: J. Liu, et al.; Environ. Sci. Technol. 55, 12984 (2021), Abstract;
Rapid changes in the phytoplankton community of a subtropical, shallow, hypereutrophic lake during the rainy season: O. Diaz-Torres, et al.; Front. Microbiol. 12, 617151 (2021), Abstract; Full Text
Microcystins in European Noble Crayfish Astacus astacus in Lake Steinsfjorden, a Planktothrix-Dominated Lake: I. A. Samdal, et al.; Toxins (Basel) 12, 298 (2020), Abstract; Full Text
Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field: ecotoxicological measurements in real environment with real microcystin exposure: A.Z. Ujvarosi, et al.; Sci. Total Environ. 662, 735 (2019), Application(s): ELISA using water bloom, water, and plant samples, Abstract;
Impacts of hydrogen peroxide and copper sulfate on the control of Microcystis aeruginosa and MC-LR and the inhibition of MC-LR degrading bacterium: M.M. Kansole, et al.; Water 9, 255 (2017), Full Text
Microcystin-LR biodegradation by Bacillus sp.: reaction rates and possible genes involved in the degradation: M.M. Kansole, et al.; Water 8, 508 (2016), Full Text
Isolation of toxic marine cyanobacteria and detection of microcystins in Thermaikos Gulf in Central Macedonia in Greece: M. Kalaitzidou, et al.; HAICTA 2015 (Proceedings of the 7th International Conference on Information and Communication Technologies in Agriculture, Food and Environment) 832 (2015), Full Text
Effect of irrigation with lake water containing microcystins on microcystin content and growth of ryegrass, clover, rape, and lettuce: J.R. Crush, et al.; Environ. Toxicol. 23, 246 (2008), Abstract;
A review of analytical methods for assessing the public health risk from microcystin in the aquatic environment: P.R. Hawkins, et al.; J. Water Supply 54, 509 (2005),
Congener-independent immunoassay for microcystins and nodularins: W.J. Fischer, et al.; Environ. Sci. Technol. 35, 4849 (2001), Abstract;
