High sensitivity ELISA, detecting low levels of carbonylated proteins
Validated in various sample types
Fully quantitative results that surpass semi-quantitative Western blot analysis
The Protein carbonyl ELISA kit is an antibody-based detection set. It has higher sensitivity, lower background, is less labour-intensive and handles more samples per day as compared to colorimetric assays.
Standard curve of Protein carbonyl ELISA kit
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Product Details
Assay Time:
4.5 hours
Applications:
ELISA
Application Notes:
For quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.
Wavelength:
450 nm
Species reactivity:
Species independent
Quantity:
96 wells (~80 tests)
Use/Stability:
Remove box containing components G, K, and L and store at -20°C! Remove box containing components H and I and store at 4°C! DO NOT FREEZE! Store the remaining of the kit at room temperature. The kit is stable at room temperature for up to 6 weeks.
Handling:
Do not freeze.
Shipping:
Ambient Temperature
Scientific Background:
Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury. The quantity of protein carbonyls in a protein sample can be determined by derivatising with dinitrophenylhydrazine (DNP) and measure bound DNP colorimetrically or immunologically. The ELISA method enables carbonyls to be measured quantitatively with microgram quantities of protein.
Cardiovascular and Blood Oxidative Stress Responses to Exercise and Acute Woodsmoke Exposure in Recreationally Active Individuals: C.M. Williamson-Reisdorph, et al.; Wilderness Environ. Med. 33, 17-31 (2022), Abstract;
Repurposing Ivermectin to augment chemotherapy’s efficacy in osteosarcoma: B. Hu, et al.; Hum. Exp. Toxicol. 41, 09603271221143693 (2022), Abstract;
Preliminary experience on the use of sucrosomial iron in hemodialysis: focus on safety, hemoglobin maintenance and oxidative stress: F. Reggiani, et al.; Int. Urol. Nephrol. 10, 11255 (2021), Abstract;
A longitudinal study of neurotrophic, oxidative, and inflammatory markers in first-onset depression in midlife women: M.A. Pasquali, et al.; Eur. Arch. Psychiatry Clin. Neurosci. 268, 771 (2018), Application(s): ELISA using human serum, Abstract;
Antibiotic bedaquiline effectively targets growth, survival and tumor angiogenesis of lung cancer through suppressing energy metabolism: X. Wu, et al.; Biochem. Biophys. Res. Commun. 495, 267 (2018), Application(s): Human lung cancer cells, Abstract;
Antibiotic drug piperacillin induces neuron cell death through mitochondrial dysfunction and oxidative damage: S. Jiang, et al.; Can. J. Physiol. Pharmacol. 96, 562 (2018), Application(s): SH-SY5Y cell lysates, Abstract;
Plasma protein carbonylation in haemodialysed patients: focus on diabetes and gender: G. Colombo, et al.; Oxid. Med. Cell. Longev. 2018, 4149681 (2018), Application(s): ELISA using human plasma, Abstract; Full Text
The biological age linked to oxidative stress modifies breast cancer aggressiveness: M.D.M. Saez-Freire, et al.; Free Radic. Biol. Med. 120, 133 (2018), Application(s): ELISA using liver homogenates, Abstract;
Tigecycline as a dual inhibitor of retinoblastoma and angiogenesis via inducing mitochondrial dysfunctions and oxidative damage: Y. Xiong, et al.; Sci. Rep. 8, 11747 (2018), Abstract; Full Text
Antibiotic tigecycline enhances cisplatin activity against human hepatocellular carcinoma through inducing mitochondrial dysfunction and oxidative damage: J. Tan, et al.; Biochem. Biophys. Res. Commun. 483, 17 (2017), Application(s): HCC cell lysates, Abstract;
Induction of Mitochondrial Dysfunction and Oxidative Damage by Antibiotic Drug Doxycycline Enhances the Responsiveness of Glioblastoma to Chemotherapy: Q. Tan, et al.; Med. Sci. Monit. 23, 4117 (2017), Application(s): Human glioblastoma cell lines A172 and U87 lysates, Abstract; Full Text
Targeting mitochondrial respiration selectively sensitizes pediatric acute lymphoblastic leukemia cell lines and patient samples to standard chemotherapy: X. Fu, et al.; Am. J. Cancer Res. 7, 2395 (2017), Abstract;
Impact of chromium dinicocysteinate supplementation on inflammation, oxidative stress, and insulin resistance in type 2 diabetic subjects: an exploratory analysis of a randomized, double-blind, placebo-controlled study: Z.M. Saiyed, et al.; Food Nutr. Res. 60, 31762 (2016), Application(s): Measurement of biomarkers in plasma, Abstract; Full Text
Pre-or post-ischemic bilirubin ditaurate treatment reduces oxidative tissue damage and improves cardiac function: B. Bakrania, et al.; Int. J. Cardiol. 202, 27 (2016), Application(s): ELISA using rat myocardial tissue, Abstract;
Analysis of the correlations between oxidative stress, gelatinases and their tissue inhibitors in the human subjects with obstructive sleep apnea syndrome: E. Hopps, et al.; J. Physiol. Pharmacol. 66, 803 (2015), Application(s): ELISA using human plasma, Abstract;
The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage: R. Shafiei, et al.; Microb. Cell. Fact. 13, 26 (2014), Application(s): EIA using bacterial cells, Abstract; Full Text
In African American type 2 diabetic patients, is vitamin D deficiency associated with lower blood levels of hydrogen sulfide and cyclic adenosine monophosphate, and elevated oxidative stress?: S.K. Jain, et al.; Antioxid. Redox Signal. 18, 1154 (2013), Application(s): ELISA using human plasma, Abstract;
Oxidative Stress and Erythrocyte Membrane Alterations in Children with Autism: Correlation with Clinical Features: A. Ghezzo, et al.; PLoS One 8, e66418 (2013), Application(s): ELISA using human plasma, Abstract; Full Text
Protein oxidation in a group of subjects with metabolic syndrome: G. Caimi, et al.; Diabetes Metab. Syndr. 7, 38 (2013), Application(s): ELISA using human plasma, Abstract;
Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers: D. Kurepa, et al.; Neonatology 102, 163 (2012), Application(s): ELISA using human plasma, Abstract; Full Text
Alteration of antioxidant defense status precedes humoral immune response abnormalities in macrosomia: M. Haddouche, et al.; Med. Sci. Monit. 17, CR650 (2011), Application(s): EIA using human blood , Abstract; Full Text
Biomarkers of oxidation, inflammation and cartilage degradation in osteoarthritis patients undergoing sulfur-based spa therapies: S. Benedetti, et al.; Clin. Biochem. 43, 973 (2010), Application(s): Carbonyls were evaluated, Abstract;
Poor maternal nutrition leads to alterations in oxidative stress, antioxidant defense capacity, and markers of fibrosis in rat islets: potential underlying mechanisms for development of the diabetic phenotype in later life: J.L. Tarry-Adkins, et al.; FASEB J. 24, 2762 (2010), Application(s): Protein carbonyl measurement was performed on serum samples, Abstract; Full Text
Atorvastatin restores endothelial function in offspring of protein-restricted rats in a cholesterol-independent manner: C. Torrens, et al.; Hypertension 53, 661 (2009), Application(s): Hepatic protein carbonyl concentration was determined, Abstract; Full Text
Endothelial dysfunction and reduced antioxidant protection in an animal model of the developmental origins of cardiovascular disease: J. Rodford, et al.; J. Physiol. 586, 4709 (2008), Application(s): EIA using rat liver tissue, Abstract; Full Text
Silencing of peroxiredoxin 3 and peroxiredoxin 5 reveals the role of mitochondrial peroxiredoxins in the protection of human neuroblastoma SH-SY5Y cells toward MPP+: S. De Simoni, et al.; Neurosci. Lett. 433, 219 (2008), Application(s): Protein carbonyl contents were assayed, Abstract;
Activated neutrophils inhibit nucleotide excision repair in human pulmonary epithelial cells: role of myeloperoxidase: N. Gungor, et al.; FASEB J. 21, 2359 (2007), Application(s): Protein carbonyl concentrations were determined by ELISA, Abstract; Full Text
Vitamin C deficiency in weanling guinea pigs: differential expression of oxidative stress and DNA repair in liver and brain: J. Lykkesfeldt, et al.; Br. J. Nutr. 98, 1116 (2007), Application(s): Protein carbonyls were measured using ELISA, Abstract; Full Text
Oxidative damage in eelpout (Zoarces viviparus), measured as protein carbonyls and TBARS, as biomarkers: B.C. Almroth, et al.; Aquat. Toxicol. 73, 171 (2005), Application(s): Protein carbonyls were measured using a sensitive ELISA, Abstract;
Oxidative stress and high density lipoprotein function in type 1 diabetes and end stage renal disease: G. Kalogerakis, et al.; Clin. Sci. (Lond) 108, 497 (2005), Application(s): Plasma protein carbonyls were determined by ELISA, Abstract;
Oxidative stress is increased in critically ill patients with acute renal failure: J. Himmelfarb, et al.; J. Am. Soc. Nephrol. 15, 2449 (2004), Abstract; Full Text
Elevated protein carbonyls as plasma markers of oxidative stress in acute pancreatitis: C.C. Winterbourn, et al.; Pancreatology 3, 375 (2003), Abstract;
Protein carbonyl groups as biomarkers of oxidative stress: I. Dalle-Donne, et al.; Clin. Chim. Acta 329, 23 (2003), Application(s): ELISA to measure plasma protein, Abstract;
Protein oxidative stress in dialysis patients: T. Donate, et al.; Adv. Perit. Dial. 18, 15 (2002), Application(s): Carbonyl protein plasma levels were measured by ELISA, Abstract;
Elevated protein carbonyls and lipid peroxidation products correlating with myeloperoxidase in tracheal aspirates from premature infants: I.H. Buss, et al.; Pediatr. Res. 47, 640 (2000), Application(s): Protein carbonyl analyses by ELISA, Abstract; Full Text
Protein carbonyl measurements show evidence of early oxidative stress in critically ill patients: C.C. Winterbourn, et al.; Crit. Care Med. 28, 143 (2000), Application(s): Plasma and bronchoalveolar lavage fluid analyzed using ELISA, Abstract;
Protein carbonyls and lipid peroxidation products as oxidation markers in preterm infant plasma: associations with chronic lung disease and retinopathy and effects of selenium supplementation: C.C. Winterbourn, et al.; Pediatr. Res. 48, 84 (2000), Application(s): Protein carbonyls were determined by an ELISA, Abstract; Full Text
Protein carbonyl measurement by a sensitive ELISA method: H. Buss, et al.; Free Radic. Biol. Med. 23, 361 (1997), Application(s): ELISA for Protein Carbonyl measurement, Abstract;
