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Protein carbonyl ELISA kit

High senitivity ELISA for quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.
 
ALX-850-312-KI01 96 wells 687.00 USD
Do you need bulk/larger quantities?
 
  • High sentitive ELISA, detecting low levels of carbonylated proteins
  • Validated in various sample types
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The Protein carbonyl ELISA kit is an antibody-based detection set. It has higher sensitivity, lower background, is less labour-intensive and handles more samples per day as compared to colorimetric assays.

Product Specification

Assay Time:4.5 hours
 
Applications:ELISA
 
Application Notes:For quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.
 
Species reactivity:Species independent
 
Quantity:96 wells (~80 tests)
 
Use/Stability:Remove box containing components G, K, and L and store at -20°C! Remove box containing components H and I and store at 4°C! DO NOT FREEZE! Store the remaining of the kit at room temperature. The kit is stable at room temperature for up to 6 weeks.
 
Handling:Do not freeze.
 
Shipping:Shipped on Blue Ice
 
Scientific Background:Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury. The quantity of protein carbonyls in a protein sample can be determined by derivatising with dinitrophenylhydrazine (DNP) and measure bound DNP colorimetrically or immunologically. The ELISA method enables carbonyls to be measured quantitatively with microgram quantities of protein.
 

Product Literature References

Impact of chromium dinicocysteinate supplementation on inflammation, oxidative stress, and insulin resistance in type 2 diabetic subjects: an exploratory analysis of a randomized, double-blind, placebo-controlled study: Z.M. Saiyed, et al.; Food Nutr. Res. 60, 31762 (2016), Application(s): Measurement of biomarkers in plasma, Abstract; Full Text
Pre-or post-ischemic bilirubin ditaurate treatment reduces oxidative tissue damage and improves cardiac function: B. Bakrania, et al.; Int. J. Cardiol. 202, 27 (2016), Application(s): ELISA using rat myocardial tissue, Abstract;
Analysis of the correlations between oxidative stress, gelatinases and their tissue inhibitors in the human subjects with obstructive sleep apnea syndrome: E. Hopps, et al.; J. Physiol. Pharmacol. 66, 803 (2015), Application(s): ELISA using human plasma, Abstract;
The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage: R. Shafiei, et al.; Microb. Cell. Fact. 13, 26 (2014), Application(s): EIA using bacterial cells, Abstract; Full Text
In African American type 2 diabetic patients, is vitamin D deficiency associated with lower blood levels of hydrogen sulfide and cyclic adenosine monophosphate, and elevated oxidative stress?: S.K. Jain, et al.; Antioxid. Redox Signal. 18, 1154 (2013), Application(s): ELISA using human plasma, Abstract;
Oxidative Stress and Erythrocyte Membrane Alterations in Children with Autism: Correlation with Clinical Features: A. Ghezzo, et al.; PLoS One 8, e66418 (2013), Application(s): ELISA using human plasma, Abstract; Full Text
Protein oxidation in a group of subjects with metabolic syndrome: G. Caimi, et al.; Diabetes Metab. Syndr. 7, 38 (2013), Application(s): ELISA using human plasma, Abstract;
Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers: D. Kurepa, et al.; Neonatology 102, 163 (2012), Application(s): ELISA using human plasma, Abstract; Full Text
Alteration of antioxidant defense status precedes humoral immune response abnormalities in macrosomia: M. Haddouche, et al.; Med. Sci. Monit. 17, CR650 (2011), Application(s): EIA using human blood , Abstract; Full Text
Biomarkers of oxidation, inflammation and cartilage degradation in osteoarthritis patients undergoing sulfur-based spa therapies: S. Benedetti, et al.; Clin. Biochem. 43, 973 (2010), Application(s): Carbonyls were evaluated, Abstract;
Poor maternal nutrition leads to alterations in oxidative stress, antioxidant defense capacity, and markers of fibrosis in rat islets: potential underlying mechanisms for development of the diabetic phenotype in later life: J.L. Tarry-Adkins, et al.; FASEB J. 24, 2762 (2010), Application(s): Protein carbonyl measurement was performed on serum samples, Abstract; Full Text
Atorvastatin restores endothelial function in offspring of protein-restricted rats in a cholesterol-independent manner: C. Torrens, et al.; Hypertension 53, 661 (2009), Application(s): Hepatic protein carbonyl concentration was determined, Abstract; Full Text
Endothelial dysfunction and reduced antioxidant protection in an animal model of the developmental origins of cardiovascular disease: J. Rodford, et al.; J. Physiol. 586, 4709 (2008), Application(s): EIA using rat liver tissue, Abstract; Full Text
Silencing of peroxiredoxin 3 and peroxiredoxin 5 reveals the role of mitochondrial peroxiredoxins in the protection of human neuroblastoma SH-SY5Y cells toward MPP+: S. De Simoni, et al.; Neurosci. Lett. 433, 219 (2008), Application(s): Protein carbonyl contents were assayed, Abstract;
Activated neutrophils inhibit nucleotide excision repair in human pulmonary epithelial cells: role of myeloperoxidase: N. Gungor, et al.; FASEB J. 21, 2359 (2007), Application(s): Protein carbonyl concentrations were determined by ELISA, Abstract; Full Text
Vitamin C deficiency in weanling guinea pigs: differential expression of oxidative stress and DNA repair in liver and brain: J. Lykkesfeldt, et al.; Br. J. Nutr. 98, 1116 (2007), Application(s): Protein carbonyls were measured using ELISA, Abstract; Full Text
Oxidative damage in eelpout (Zoarces viviparus), measured as protein carbonyls and TBARS, as biomarkers: B.C. Almroth, et al.; Aquat. Toxicol. 73, 171 (2005), Application(s): Protein carbonyls were measured using a sensitive ELISA, Abstract;
Oxidative stress and high density lipoprotein function in type 1 diabetes and end stage renal disease: G. Kalogerakis, et al.; Clin. Sci. (Lond) 108, 497 (2005), Application(s): Plasma protein carbonyls were determined by ELISA, Abstract;
Oxidative stress is increased in critically ill patients with acute renal failure: J. Himmelfarb, et al.; J. Am. Soc. Nephrol. 15, 2449 (2004), Abstract; Full Text
Elevated protein carbonyls as plasma markers of oxidative stress in acute pancreatitis: C.C. Winterbourn, et al.; Pancreatology 3, 375 (2003), Abstract;
Protein carbonyl groups as biomarkers of oxidative stress: I. Dalle-Donne, et al.; Clin. Chim. Acta 329, 23 (2003), Application(s): ELISA to measure plasma protein, Abstract;
Protein oxidative stress in dialysis patients: T. Donate, et al.; Adv. Perit. Dial. 18, 15 (2002), Application(s): Carbonyl protein plasma levels were measured by ELISA, Abstract;
Elevated protein carbonyls and lipid peroxidation products correlating with myeloperoxidase in tracheal aspirates from premature infants: I.H. Buss, et al.; Pediatr. Res. 47, 640 (2000), Application(s): Protein carbonyl analyses by ELISA, Abstract; Full Text
Protein carbonyl measurements show evidence of early oxidative stress in critically ill patients: C.C. Winterbourn, et al.; Crit. Care Med. 28, 143 (2000), Application(s): Plasma and bronchoalveolar lavage fluid analyzed using ELISA, Abstract;
Protein carbonyls and lipid peroxidation products as oxidation markers in preterm infant plasma: associations with chronic lung disease and retinopathy and effects of selenium supplementation: C.C. Winterbourn, et al.; Pediatr. Res. 48, 84 (2000), Application(s): Protein carbonyls were determined by an ELISA, Abstract; Full Text
Protein carbonyl measurement by a sensitive ELISA method: H. Buss, et al.; Free Radic. Biol. Med. 23, 361 (1997), Application(s): ELISA for Protein Carbonyl measurement, Abstract;

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Protein carbonyl ELISA kit 

High senitivity ELISA for quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.
ELISA | Print as PDF
 
ALX-850-312-KI01 96 wells 687.00 USD
Do you need bulk/larger quantities?
 

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