High power chaperone mix which mediates the ATP-dependent refolding of aggregated proteins (i.e. inclusion bodies).
Product Specification
| Concentration: | 2.4mg/ml total protein. |
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| Quantity: | 85µl |
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| Purity: | ≥95% |
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| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 50mM sodium chloride, 5mM magnesium dichloride, 50μM EDTA and 5% (v/v) glycerol. |
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| Source/Host: | Produced in E. coli. |
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| Kit/Set Contains: | Contains the following recombinant E. coli proteins:ClpB (10µM)DnaK (10µM)DnaJ (2µM)GrpE (1µM)GroEL (10µM)GroES (10µM) |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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Product Literature References
Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network: P. Goloubinoff, et al.; PNAS
96, 13732 (1999),
Abstract;
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