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Chaperone cocktail

 
ALX-850-307-KI01 1 Kit 677.00 USD
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High power chaperone mix which mediates the ATP-dependent refolding of aggregated proteins (i.e. inclusion bodies).

Product Specification

Purity:≥95%
 
Kit/Set Contains:Contains the following recombinant E. coli proteins:
ClpB (10µM)
DnaK (10µM)
DnaJ (2µM)
GrpE (1µM)
GroEL (10µM)
GroES (10µM)
 
Quantity:85µl
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 50mM sodium chloride, 5mM magnesium dichloride, 50μM EDTA and 5% (v/v) glycerol.
 
Concentration:2.4mg/ml total protein.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Source:Produced in E. coli.
 

Product Literature References

Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network: P. Goloubinoff, et al.; PNAS 96, 13732 (1999), Abstract; Full Text

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