The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
Figure: ApoSENSOR™ Cell Viability Assay effectively detects apoptosis. Apoptosis was induced in Jurkat cells and ATP levels were analyzed according to the kit instructions. Left: Time course showing decrease in ATP levels following induction of apoptosis with camptothecin. The decrease in ATP level correlates with the increase in annexin V positive staining cells detected by Annexin V-FITC Apoptosis Detection Kit according to the kit instructions. Right: Jurkat cells were treated with various apoptosis inducers as indicated for 12 hours and ATP levels decrease dramatically for all treatments.
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