Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages: No radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay.
Product Details
| Alternative Name: | CCKi-F |
| |
| Applications: | Fluorescence microscopy
|
| |
| Handling: | Protect from light. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Technical Info/Product Notes: | Principle: The amount of a fluorescent dye, calcein, produced from calcein-AM (3’,6’-Di(O-acetyl)-2’,7’-bis[N,N-bis-(carboxymethyl)aminomethyl]fluorescein . tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lipophilic due to the acetoxymethyl groups, it can rapidly permeate into the cytoplasma through the cell membrane. The detection range of the number of viable cells by CCKi-F is from <50 to at least 25,000 cells. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Identification of novel pathways and molecules able to down-regulate PHOX2B gene expression by in vitro drug screening approaches in neuroblastoma cells: E. Di Zanni, et al.; Exp. Cell Res.
336, 43 (2015),
Application(s): Fluorescence Microscopy ,
Abstract;
