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Caspase (active) (recombinant) set lll

 
ALX-850-243-KI01 1 Set 1,105.00 USD
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Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays. 

Product Specification

Recommended Dilutions/Conditions:We recommend using 1U per assay for analyzing caspase activity.
 
Specific Activity:One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8, LEHD-pNA for caspase-9, and IETD-pNA for caspase-10) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
 
Purity:All components ≥90% (SDS-PAGE)
 
Kit/Set Contains:Contains 25 units each of the following caspases:
- Caspase-1 (active) (human) (recombinant), yellow cap
- Caspase-2 (active) (human) (recombinant), orange cap
- Caspase-3 (active) (human) (recombinant), red cap
- Caspase-6 (active) (human) (recombinant), violet cap
- Caspase-7 (active) (human) (recombinant), red cap
- Caspase-8 (active) (human) (recombinant), green cap
- Caspase-9 (active) (human) (recombinant), blue cap
- Caspase-10 (active) (human) (recombinant), amber cap
 
Formulation:Lyophilized.
 
Reconstitution:Reconstitute to 0.1-1U/µl in PBS.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Source:Produced in E. coli.
 

Product Literature References

Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice: B.K. Wong, et al.; Neurobiol. Dis. 76C, 24 (2015), Application(s): Cell Culture, Abstract;

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