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Quick apoptotic DNA ladder detection kit

ALX-850-242-KI01 50 tests 447.00 USD
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The Quick Apoptotic DNA Ladder Detection Kit offers fast and sensitive detection of DNA fragmentation in apoptotic cells. Unlike other similar kits on the market which require 1-2 days to prepare nucleic acids, the new procedure requires less than 90 minutes to prepare chromosomal DNA in a single tube, with no need for extraction or a column. DNA ladders can easily be visualized by agarose gel electrophoresis. This procedure increases recovery of small fragmented DNA, and therefore improves detection sensitivity.
Figure: Quick detection of DNA ladder in apoptotic cells. Apoptosis was induced in Jurkat cells with camtothecin (2µM) for 0 hours (lane1), 6 hours (lane2) and 12 hours (lane3).  Chromosomal DNA was prepared using the Quick Apoptotic DNA Ladder Detection Kit according to the kit instructions. 20µl of each sample was electrophoresed on a 1.2% agarose/EtBr gel.
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Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C

Product Literature References

Hepatic CYP3A suppression by high concentrations of proteasomal inhibitors: a consequence of endoplasmic reticulum (ER) stress induction, activation of RNA-dependent protein kinase-like ER-bound eukaryotic initiation factor 2alpha (eIF2alpha)-kinase (PERK) and general control nonderepressible-2 eIF2alpha kinase (GCN2), and global translational shutoff: P. Acharya, et al.; Mol. Pharmacol. 76, 503 (2009), Abstract; Full Text

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