Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy and in apoptotic cells. In healthy cells, MitoCapture™ accumulates and aggregates in the mitochondria, giving them a bright red fluorescence. In apoptotic cells, MitoCapture™ cannot aggregate in the mitochondria due to the altered mitochondrial membrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using the FITC channel for green monomers (Ex(max): 488nm; Em(max): 530nm + 30nm), and the PI channel for red aggregates (Ex(max): 488nm; Em(max): 590nm + 42nm). An assay is completed within 20-30 min.
MitoCapture™ (Prod. No. ALX-850-232
) effectively detects disruption of mitochondrial transmembrane potential in apoptotic cells. Apoptosis was induced in Jurkat cells by 2mM camptothecin for various hours as indicated. Cells were incubated with MitoCapture for 15 min. Results were analyzed by flow cytometry using the FITC channel.
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|Applications:||Flow Cytometry, Fluorescence microscopy, Fluorescent detection|
|Handling:||Store Incubation Buffer at 4°C after opening. Protect from light. Avoid freeze/thaw cycles.|
|Shipping:||Shipped on Blue Ice|
|Long Term Storage:||-20°C|
|Kit/Set Contains:||MitoCapture™ ReagentIncubation Buffer.|
Product Literature References
TANK2, a new TRF1-associated poly(ADP-ribose) polymerase, causes rapid induction of cell death upon overexpression
: P.G. Kaminker, et al.; J. Biol. Chem. 276
, 35891 (2001), Abstract
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