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MitoCapture™ Mitochondrial apoptosis detection kit

 
ALX-850-232-KI01 25 tests 190.00 USD
 
ALX-850-232-KI02 100 tests 413.00 USD
 
Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy and in apoptotic cells. In healthy cells, MitoCapture™ accumulates and aggregates in the mitochondria, giving them a bright red fluorescence.
850-232
Figure: MitoCapture™  (Prod. No. ALX-850-232) effectively detects disruption of mitochondrial transmembrane potential in apoptotic cells. Apoptosis was induced in Jurkat cells by 2mM camptothecin for various hours as indicated. Cells were incubated with MitoCapture for 15 min. Results were analyzed by flow cytometry using the FITC channel.
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850-232

Product Specification

Kit/Set Contains:MitoCapture™ ReagentIncubation Buffer.
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
Protect from light.
Store Incubation Buffer at 4°C after opening.
 

Product Literature References

TANK2, a new TRF1-associated poly(ADP-ribose) polymerase, causes rapid induction of cell death upon overexpression: P.G. Kaminker, et al.; J. Biol. Chem. 276, 35891 (2001), Abstract; Full Text

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