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MitoCapture™ Mitochondrial apoptosis detection kit

 
ALX-850-232-KI01 25 tests 209.00 USD
 
ALX-850-232-KI02 100 tests 447.00 USD
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Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy and in apoptotic cells. In healthy cells, MitoCapture™ accumulates and aggregates in the mitochondria, giving them a bright red fluorescence. In apoptotic cells, MitoCapture™ cannot aggregate in the mitochondria due to the altered mitochondrial membrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using the FITC channel for green monomers (Ex(max): 488nm; Em(max): 530nm + 30nm), and the PI channel for red aggregates (Ex(max): 488nm; Em(max): 590nm + 42nm). An assay is completed within 20-30 min.
850-232
Figure: MitoCapture™  (Prod. No. ALX-850-232) effectively detects disruption of mitochondrial transmembrane potential in apoptotic cells. Apoptosis was induced in Jurkat cells by 2mM camptothecin for various hours as indicated. Cells were incubated with MitoCapture for 15 min. Results were analyzed by flow cytometry using the FITC channel.
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850-232

Product Specification

Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection
 
Handling:Store Incubation Buffer at 4°C after opening. Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:MitoCapture™ ReagentIncubation Buffer.
 

Product Literature References

TANK2, a new TRF1-associated poly(ADP-ribose) polymerase, causes rapid induction of cell death upon overexpression: P.G. Kaminker, et al.; J. Biol. Chem. 276, 35891 (2001), Abstract; Full Text

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