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Caspase-9 colorimetric assay kit

 
ALX-850-223-KI01 25 tests 254.00 USD
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Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate LEHD-pNA.

Product Specification

Alternative Name:ICE-LAP-6, Mch6
 
Applications:Colorimetric detection
Activity assay
 
Handling:Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl LEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
 
Technical Info/Product Notes:The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-9 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
 
UniProt ID:P55211
 

Product Literature References

Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells: C.C. Chen, et al.; Drug Des. Devel. Ther. 8, 459 (2014), Abstract; Full Text
Antidiabetic drug metformin alleviates endotoxin-induced fulminant liver injury in mice: H. Yuan, et al.; Int. Immunopharmacol. 12, 682 (2012), Abstract;
Therapeutic benefits of the group B3 vitamin nicotinamide in mice with lethal endotoxemia and polymicrobial sepsis: H. Yuan, et al.; Pharmacol. Res. 65, 328 (2012), Abstract;
Seasonal study of apoptotic markers in lizard oviduct: L. Assisi, et al.; J. Exp. Zool. A Ecol. Genet. Physiol. 315, 291 (2011), Abstract;

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