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Caspase-8 colorimetric assay kit

ALX-850-221-KI01 25 tests 204.00 USD
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Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate IETD-pNA.

Product Specification

Alternative Name:FLICE, Mch5, MACH
Applications:Colorimetric detection
Activity assay
Handling:Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Contents:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl IETD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
Technical Info/Product Notes:The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
UniProt ID:Q14790

Product Literature References

Ruxolitinib combined with vorinostat suppresses tumor growth and alters metabolic phenotype in hematological diseases: M. Civallero, et al.; Oncotarget 8, 103797 (2017), Abstract; Full Text
Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells: C.C. Chen, et al.; Drug Des. Devel. Ther. 8, 459 (2014), Abstract; Full Text
Antidiabetic drug metformin alleviates endotoxin-induced fulminant liver injury in mice: H. Yuan, et al.; Int. Immunopharmacol. 12, 682 (2012), Abstract;
Therapeutic benefits of the group B3 vitamin nicotinamide in mice with lethal endotoxemia and polymicrobial sepsis: H. Yuan, et al.; Pharmacol. Res. 65, 328 (2012), Abstract;

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