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Caspase-5 colorimetric assay kit

 
ALX-850-217-KI01 25 tests 199.00 USD
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Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.

Product Specification

Alternative Name:ICH-3, Protease TY, ICErel-III
 
Applications:Colorimetric detection
Activity assay
 
Handling:Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
 
Technical Info/Product Notes:The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
 
UniProt ID:P51878
 

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