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Caspase-3 colorimetric assay kit

 
ALX-850-215-KI01 25 tests 218.00 USD
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Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate DEVD-pNA.

Product Specification

Alternative Name:CPP32, Yama, Apopain
 
Applications:Colorimetric detection
Activity assay
 
Handling:Store Lysis Buffer, Reaction Buffer and Dilution Buffer at 4°C after opening. Protect from light.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl DEVD-pNA (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
 
Technical Info/Product Notes:The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.

Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
 
UniProt ID:P42574
 

Product Literature References

Activity of BKM120 and BEZ235 against Lymphoma Cells: M. Civallero, et al.; Biomed Res. Int. 2015, Article ID 870918 (2015), Application(s): Measurement of caspase-3 activity in human lymphoma cell lines, Abstract; Full Text
Direct antiapoptotic effects of growth hormone are mediated by PI3K/Akt pathway in the chicken bursa of Fabricius: J.L. Luna-Acosta, et al.; Gen. Comp. Endocrinol. 224, 148 (2015), Application(s): Caspase-3 assay in chicken B cell lysate, Abstract;
Improving anticancer efficacy of (-)-epigallocatechin-3-gallate gold nanoparticles in murine B16F10 melanoma cells: C.C. Chen, et al.; Drug Des. Devel. Ther. 8, 459 (2014), Abstract; Full Text
Liquiritigenin induces tumor cell death through mitogen-activated protein kinase- (MPAKs-) mediated pathway in hepatocellular carcinoma cells: D. Wang, et al.; Biomed. Res. Int. 2014, Article ID 965316 (2014), Application(s): Measurement of caspase-3 activity in PLC/PRF/5 (p53 mutant) and HepG2 (p53 wild type) human tumor cells, Abstract; Full Text
A2B adenosine receptor blockade inhibits growth of prostate cancer cells: Q. Wei, et al.; Purinergic Signal. 9, 271 (2013), Application(s): Measurement of caspase-3 activity in human PC-3 cells, Abstract;
Therapeutic benefits of the group B3 vitamin nicotinamide in mice with lethal endotoxemia and polymicrobial sepsis: H. Yuan, et al.; Pharmacol. Res. 65, 328 (2012), Abstract;
Activation of the P2Y1 receptor induces apoptosis and inhibits proliferation of prostate cancer cells: Q. Wei, et al. ; Biochem. Pharmacol. 82, 418 (2011), Application(s): Measurement of caspase-3 activity in human PC-3 cells, Abstract;
Seasonal study of apoptotic markers in lizard oviduct: L. Assisi, et al.; J. Exp. Zool. A Ecol. Genet. Physiol. 315, 291 (2011), Abstract;

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