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Cell Counting Kit-8

Colorimetric kit for simple and accurate cell proliferation and cytotoxicity assays.
 
ALX-850-039-0100 100 tests 85.00 USD
 
ALX-850-039-KI01 500 tests 182.00 USD
 
ALX-850-039-KI02 5x500 tests 560.00 USD
Do you need bulk/larger quantities?
 
  • One-step, ready-to-use solution with no radioisotopes
  • High sensitivity that correlates with the [3H]-thymidine incorporation assay
  • High-throughput screening without a solubilization step
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity.

It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results.
ALX-850-039 web Fig1
ALX-850-039 Fig6
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation. CCK-8 shows greatest sensitivity.
ALX-850-039 Fig7
CTLL-2 cells were incubated with various concentrations of IL-2 for 72 hours. CCK-8 solution was added to each well and the absorbance at 450nm was measured. IL-2 exposure resulted in an increase absorbance which correlates to an increase in cell proliferation.
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ALX-850-039 web Fig1 ALX-850-039 Fig6 ALX-850-039 Fig7

Product Specification

Alternative Name:CCKi-8
 
Applications:Colorimetric detection
 
Quantity:100 tests: 1 mL
500 tests: 5 mL
2500 tests: 5 x 5 mL
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:WST-8 solution, 1-methoxy-PMS
 
Technical Info/Product Notes:

Principle: Employs the tetrazolium salt WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium . monosodium salt), that produces a highly water soluble formazan dye upon biochemical reduction in the presence of an electron carrier, 1-methoxy-PMS. The amount of the yellow colored formazan dye generated by dehydrogenases in cells is directly proportional to the number of viable cells in a culture medium.

 

Product Literature References

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Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture: F. Angelini, et al.; Bioimpacts 6, 15 (2016), Application(s): WST-assay (cell proliferation and cytotoxicity assays), Abstract; Full Text
In vitro inhibition of Toxoplasma gondii by the anti-malarial candidate, 6-(1,2,6,7-tetraoxaspiro[7.11]nonadec-4-yl)hexan-1-ol: C.F. Xin, et al.; Parasitol. Int. 16, 30212 (2016), Application(s): Cell viability analysis to measure anti-T. gondii activity and cytotoxicity in vitro, Abstract;
Inhibitory effect of quercetin on colorectal lung metastasis through inducing apoptosis, and suppression of metastatic ability: J.Y. Kee, et al.; Phytomedicine 23, 1680 (2016), Application(s): WST assay, mouse colon carcinoma cell line , Abstract;
The level of nitric oxide regulates lipocalin-2 expression under inflammatory condition in RINm5F beta-cells: S.Y. Chang, et al.; Biochem. Biophys. Res. Commun. 476, 7 (2016), Application(s): Cell viability assay, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Cell viability assay; Effect of VD2 and Aβ25–35 induction on BV2 cells, Abstract;
ZEB1 is neither sufficient nor required for epithelial-mesenchymal transition in LS174T colorectal cancer cells: S. Jägle, et al.; Biochem. Biophys. Res. Commun. (2016), Abstract;
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells: J.H. Kim, et al.; Int. J. Nanomedicine 10, 5513 (2015), Application(s): Cytotoxicity measurement, Abstract; Full Text
Circulating Pneumolysin Is a Potent Inducer of Cardiac Injury during Pneumococcal Infection: Y. Alhamdi, et al.; PLoS Pathog. 11, e1004836 (2015), Application(s): Assay using murine cardiomyocytes, Abstract; Full Text
Fabrication of Duck's feet collagen-Silk hybrid biomaterial for Tissue Engineering: S.H. Kim, et al.; Int. J. of Biol. Macromol. 85, 442 (2015), Application(s): Cytotoxicity test, Abstract;
Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells: Y.C. Yoon, et al.; Molecules 20, 22128 (2015), Application(s): Determined the viability of cells, Abstract; Full Text
ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells: S.G. Tilson, et al.; PLoS One 10, e0132823 (2015), Application(s): Toxicity Assay, Abstract; Full Text
The anti-inflammatory effect of prunus yedoensis bark extract on adipose tissue in diet-induced obese mice: H. Kang, et al.; Evid. Based Complement. Alternat. Med. 2015, Article ID 937904 (2015), Application(s): Cell viability assay, Abstract; Full Text
3D silk fibroin scaffold incorporating titanium dioxide (TiO2) nanoparticle (NPs) for tissue engineering: J.H. Kim, et al.; Int. J. Biol. Macromol. 68, 158 (2014), Abstract;
Dietary fat sources differentially modulate intestinal barrier and hepatic inflammation in alcohol-induced liver injury in rats: W. Zhong, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 305, G919 (2013), Abstract; Full Text
Hypoxia-inducible factor (HIF)-1α suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis and bone destruction: P. Storti, et al.; Leukemia 27, 1697 (2013), Abstract;
Solomonsterol A, a marine pregnane-X-receptor agonist, attenuates inflammation and immune dysfunction in a mouse model of arthritis: A. Mencarelli, et al.; Mar. Drugs 12, 36 (2013), Abstract; Full Text
Increased osteocyte death in multiple myeloma patients: role in myeloma-induced osteoclast formation: N. Giuliani, et al.; Leukemia 26, 1391 (2012), Abstract;
Biobased poly(propylene sebacate) as shape memory polymer with tunable switching temperature for potential biomedical applications: B. Guo, et al.; Biomacromolecules 12, 1312 (2011), Abstract; Full Text
Circulating Toll-like receptor (TLR) 2, TLR4, and regulatory T cells in patients with chronic hepatitis C: J.P. Wang, et al.; APMIS 118, 261 (2010), Abstract;
Effect of proteasome inhibitors with different chemical structures on the ubiquitin-proteasome system in vitro: V. Csizmadia, et al.; Vet. Pathol. 47, 358 (2010), Abstract;
Markers of tumor-initiating cells predict chemoresistance in breast cancer: C. Gong, et al.; PLoS One 5, e15630 (2010), Abstract; Full Text
Overexpression of Toll-like receptor 2/4 on monocytes modulates the activities of CD4(+)CD25(+) regulatory T cells in chronic hepatitis B virus infection: Y. Zhang, et al.; Virology 397, 34 (2010), Abstract;
Synthesis, properties and in vitro photodynamic activity of water-soluble azaphthalocyanines and azanaphthalocyanines: P. Zimcik, et al.; Photochem. Photobiol. 86, 168 (2010), Abstract;
COX-1 is coupled with mPGES-1 and ABCC4 in human cervix cancer cells: H. Radilova, et al.; Mol. Cell Biochem. 330, 131 (2009), Abstract;
Interleukin-1beta increases gap junctional communication among synovial fibroblasts via the extracellular-signal-regulated kinase pathway: C. Niger, et al.; Biol. Cell 102, 37 (2009), Abstract; Full Text
Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect: Y. Kim, et al.; J. Biol. Chem. 280, 23273 (2005), Abstract; Full Text
Cyclin G2 dysregulation in human oral cancer: Y. Kim, et al.; Cancer Res. 64, 8980 (2004), Abstract; Full Text
Mitogen-activated protein kinases mediate peroxynitrite-induced cell death in human bronchial epithelial cells: E. Nabeyrat, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 284, L1112 (2003), Abstract; Full Text
Increased cytotoxicity of cadmium in fibroblasts lacking c-fos: M. Matsuoka, et al.; Biochem. Pharmacol. 59, 1573 (2000), Abstract;
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