Annexin V-Biotin apoptosis detection kit



ALX-850-031-KI02	100 tests	363.00 USD

ALX-850-031-KI01	300 tests	547.00 USD

Biotin-labelled recombinant human annexin V (produced in E. coli) shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm). Exhibits anti-phospholipase activity and binds to phosphatidylserine.

Product Specification

Purity:	≥98% (SDS-PAGE; HPLC)

Kit/Set Contains:	Annexin V-Biotin: 100 tests/500µl; 300 tests/1.5ml;Liquid. Contains 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide. Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl₂. Propidium Iodide: 1.6ml; 20µg/ml.

Application:	For the detection of phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells by flow cytometry.

Long Term Storage:	+4°C

Handling:	Avoid freeze/thaw cycles. After opening annexin V-Biotin, aliquot contents and freeze at -20°C.

Background / Technical Information:	UniProt ID P08758: Annexin V (human) AfCS Signaling Gateway ID A000281 : Annexin V (mouse)

Protocol:	Flow Cytometry protocol: - Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water) - Brief centrifugation of annexin V-Biotin before use is recommended - Wash cells in PBS by gentle shaking or pipetting up and down. - Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x10⁵/ml - Take 195µl cell suspension and add 5µl annexin V-Biotin - Mix and incubate 15 minutes at room temperature - Wash cells 2x in prediluted binding buffer - Resuspend in prediluted binding buffer - Add Streptavidin-FITC - Mix and incubate 30 minutes in the dark at room temperature - Wash cells 1x in prediluted binding buffer - Resuspend in 190µl prediluted binding buffer - Add 10µl of the 20µg/ml propidium iodide stock solution (end concentration 1µg/ml) - FACS analysis