| ALX-850-031-KI02 | 100 tests | 352.00 USD | ![]() |
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| ALX-850-031-KI01 | 300 tests | 531.00 USD | ![]() |
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| Purity: | ≥98% (SDS-PAGE; HPLC) |
| Kit/Set Contains: | Annexin V-Biotin: 100 tests/500µl; 300 tests/1.5ml;Liquid. Contains 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide. Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl2. Propidium Iodide: 1.6ml; 20µg/ml. |
| Application: | For the detection of phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells by flow cytometry. |
| Shipping: | SHIPPED ON BLUE ICE |
| Long Term Storage: | +4°C |
| Handling: | Avoid freeze/thaw cycles. After opening annexin V-Biotin, aliquot contents and freeze at -20°C. |
| Background / Technical Information: | UniProt link P08758: Annexin V (human) AfCS Signalling Gateway link A000281 : Annexin V (mouse) Flow Cytometry protocol:- Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)- Brief centrifugation of annexin V-Biotin before use is recommended- Wash cells in PBS by gentle shaking or pipetting up and down.- Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x105/ml- Take 195µl cell suspension and add 5µl annexin V-Biotin- Mix and incubate 15 minutes at room temperature- Wash cells 2x in prediluted binding buffer- Resuspend in prediluted binding buffer- Add Streptavidin-FITC- Mix and incubate 30 minutes in the dark at room temperature- Wash cells 1x in prediluted binding buffer- Resuspend in 190µl prediluted binding buffer- Add 10µl of the 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)- FACS analysis |
