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Phosphotyrosine detection set

 
ALX-850-025-KI01 1 Set 596.00 USD
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This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.
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Figure: Western blot analysis of whole cell lysates of pervanadate-treated A431 cells. Immunoblots were probed with the indicated MAbs for 1h at 15-22°C and developed wit ECL (exposure time: 15 sec).M: Molecular Weight Standard1: Clone 1F9, 0.5μg/ml (Prod. No. ALX-804-671)2: Clone 2A5, 0.2μg/ml (Prod. No. ALX-804-670)3: Clone 2C8, 0.2μg/ml (Prod. No. ALX-804-171) 4: Clone 3B12, 0.2μg/ml (Prod. No. ALX-804-172) 5: Clone 9H8, 0.2μg/ml (Prod. No. ALX-804-173) 6: Clone 16F4, 0.2μg/ml (Prod. No. ALX-804-174)7: Clone 9F1, 0.2μg/ml (Prod. No. ALX-804-669)

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Product Specification

Applications:IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Kit/Set Contains:Contains a positive control for immunoblot applications as well as 25µg each of the following MAbs:
Clone 2C8
Clone 3B12
Clone 9H8
Clone 16F4 (Prod. No. ALX-804-174)
Clone 9F1 (Prod. No. ALX-804-669)
Clone 2A5 (Prod. No. ALX-804-670)
Clone 1F9 (Prod. No. ALX-804-671)
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Antibodies directed against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.
 

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Phosphotyrosine monoclonal antibody (16F4) 

ELISA, IP, WB | Print as PDF
 
ALX-804-174-C100 100 µg 323.00 USD
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