| ALX-850-025-KI01 | 1 Set | 767.00 USD |  |
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Figure: Western blot analysis of whole cell lysates of pervanadate-treated A431 cells. Immunoblots were probed with the indicated MAbs for 1h at 15-22°C and developed wit ECL (exposure time: 15 sec).M: Molecular Weight Standard1: Clone 1F9, 0.5μg/ml (Prod. No. ALX-804-671)2: Clone 2A5, 0.2μg/ml (Prod. No. ALX-804-670)3: Clone 2C8, 0.2μg/ml (Prod. No. ALX-804-171) 4: Clone 3B12, 0.2μg/ml (Prod. No. ALX-804-172) 5: Clone 9H8, 0.2μg/ml (Prod. No. ALX-804-173) 6: Clone 16F4, 0.2μg/ml (Prod. No. ALX-804-174)7: Clone 9F1, 0.2μg/ml (Prod. No. ALX-804-669)
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| Applications: | IP, WB |
| Recommended Dilutions/Conditions: | Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Contents: | Contains a positive control for immunoblot applications as well as 25µg each of the following MAbs: Clone 2C8 Clone 3B12 Clone 9H8 Clone 16F4 (Prod. No. ALX-804-174) Clone 9F1 (Prod. No. ALX-804-669) Clone 2A5 (Prod. No. ALX-804-670) Clone 1F9 (Prod. No. ALX-804-671) |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | Antibodies directed against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. |
| Regulatory Status: | RUO - Research Use Only |
Phosphotyrosine monoclonal antibody (16F4) |
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| ELISA, IP, WB | Print as PDF | ||||||
| ALX-804-174-C100 | 100 µg | 435.00 USD | |
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