Replaces Prod. #: ADI-ADK-700
FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).
Typical flow cytometry results. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.
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Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy, Fluorescent detection
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| Application Notes: | For the detection of apoptotic cells by flow cytometry or microscopy |
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| Purity: | ≥98% (SDS-PAGE; HPLC) |
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| Handling: | After opening annexin V-FITC, aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml;Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl2.
Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml). |
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| Protocol: | Flow Cytometry Protocol:
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Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
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Brief centrifugation of annexin V-FITC before use is recommended
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Wash cells in PBS by gentle shaking or pipetting up and down.
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Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x105/ml
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Take 195µl cell suspension and add 5µl annexin V-FITC
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Mix and incubate 10 minutes in the dark at room temperature
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Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
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Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
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FACS analysis
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| Regulatory Status: | RUO - Research Use Only |
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GX15-070 (Obatoclax) Induces Apoptosis and Inhibits Cathepsin D- and L–Mediated Autophagosomal Lysis in Antiestrogen-Resistant Breast Cancer Cells : J.L. Schwartz-Roberts, et al.; Mol. Cancer Ther.
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