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Annexin V-FITC apoptosis detection kit

Easy, quick assay to distinguish apoptotic cells vs necrotic cells
 
ALX-850-020-KI02 100 tests 383.00 USD
 
ALX-850-020-KI01 300 tests 566.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ADI-ADK-700

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).
ALX-850-020 flow
Typical flow cytometry results. The Annexin V-FITC apoptosis detection kit allows the detection and discrimination between apoptotic and necrotic cells.
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ALX-850-020 flow

Product Specification

Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection
 
Application Notes:For the detection of apoptotic cells by flow cytometry or microscopy
 
Purity:≥98% (SDS-PAGE; HPLC)
 
Handling:After opening annexin V-FITC, aliquot contents and freeze at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml;Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Binding Buffer: 50ml (4x concentrated)Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl2.
Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml).
 
Protocol:Flow Cytometry Protocol:
  1. Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
  2. Brief centrifugation of annexin V-FITC before use is recommended
  3. Wash cells in PBS by gentle shaking or pipetting up and down.
  4. Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x105/ml
  5. Take 195µl cell suspension and add 5µl annexin V-FITC
  6. Mix and incubate 10 minutes in the dark at room temperature
  7. Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
  8. Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
  9. FACS analysis
 

Product Literature References

Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study: P. Randelli, et al.; BMC Complement. Altern. Med. 16, 293 (2016), Application(s): Cell apoptosis analysis, PST and control cells, Abstract; Full Text
Nanoparticles of barium induce apoptosis in human phagocytes: L. Mores, et al.; Int. J. Nanomedicine 10, 6021 (2015), Application(s): Flow cytometry apoptosis assay with human cells, Abstract; Full Text
Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy: D. Park, et al.; Evid. Based Complement. Alternat. Med. 2015, 764251 (2015), Application(s): Flow Cytometry, Abstract; Full Text
GX15-070 (Obatoclax) Induces Apoptosis and Inhibits Cathepsin D- and L–Mediated Autophagosomal Lysis in Antiestrogen-Resistant Breast Cancer Cells : J.L. Schwartz-Roberts, et al.; Mol. Cancer Ther. 12, 448 (2013), Abstract;
Glucose-Regulated Protein 78 Controls Cross-talk between Apoptosis and Autophagy to Determine Antiestrogen Responsiveness: K. Cook, et al.; Cancer Res. 72, 3337 (2012), Abstract; Full Text
Selective FLT3 inhibition of FLT3-ITD+ acute myeloid leukaemia resulting in secondary D835Y mutation: a model for emerging clinical resistance patterns: A. Moore, et al.; Leukemia 26, 1462 (2012), Abstract; Full Text
Specific binding of hypochlorite-oxidized HDL to platelet CD36 triggers proinflammatory and procoagulant effects: A. Assinger, et al.; Atherosclerosis 212, 153 (2010), Abstract;

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Multiplex assay that distinguishes between  healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other green fluorescent probes
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
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Do you need bulk/larger quantities?
 

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