Annexin V-FITC apoptosis detection kit

ALX-850-020

Revised 01-Feb-10

Annexin V-FITC apoptosis detection kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-020-KI02		100 tests	352.00 USD
ALX-850-020-KI01		300 tests	531.00 USD

Product Specification

KIT/SET CONTAINS:	Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml; Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide. Binding Buffer: 50ml (4x concentrated) Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl_2. Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml).
PURITY:	≥98% (SDS-PAGE; HPLC)
APPLICATION:	For the detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After opening annexin V-FITC, aliquot contents and freeze at -20°C.

Product Description

FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:

- Dilute binding buffer 1:4 in distilled water (50ml binding buffer and 150ml water)
- Brief centrifugation of annexin V-FITC before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down.
- Resuspend cells in prediluted binding buffer; adjust cell density to 2-5x10⁵/ml
- Take 195µl cell suspension and add 5µl annexin V-FITC
- Mix and incubate 10 minutes in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl prediluted binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
- FACS analysis

Further Categories Containing This Product:

Detection Kits