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LMP-1 (Epstein-Barr virus) clonal antibody (D24-G)

 
ALX-810-231-R500 500 µl 647.00 USD
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Product Specification

Alternative Name:Latent Membrane Protein-1, EBV
 
Clone:D24-G
 
Host:Rabbit
 
Isotype:IgG
 
Immunogen:Synthetic peptide corresponding to the internal region of Epstein-Barr virus, LMP-1 (latent membrane protein-1).
 
UniProt ID:P03230
 
Species reactivity:Epstein-Barr virus
 
Specificity:Recognizes Epstein-Barr virus, LMP-1.
 
Applications:IHC (FS), IHC (PS)
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100-1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
 
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
 
Handling:Centrifuge the vial before opening the cap. After opening, prepare aliquots and store at +4°C.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
 
Protocol:IHC (paraffin sections) Protocol:
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each.
3. Rinse in distilled water.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water for 5 minutes.
6. For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in water bath at 96°C for 40 minutes. (Alternatively adjust your own protocol, keeping the required pH.)
7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes.
8. Rinse distilled water.
9. Wash in 0.05M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100- 1:200 for 1 hour in the closed wet chamber.
11. Wash twice 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB).
13. Wash twice 5 minutes with buffer A.
14. Apply the chromogen (DAB), 10 minutes.
15. Wash in water for 10 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in water for 10 minutes.
18. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each.
19. Wash the section in 2 changes of xylene for 2 minutes each.
20. Mount the slide for observation.

*Citrate Buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0):
Citric acid (anhydrous)----------1.92g
Distilled water---------------------1000ml
Mix to dissolve in 700ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at 4°C for longer storage.
 
810-231
Figure: Formalin-fixed and paraffin-embedded human lymph node tissue (4µm) corresponding to Hodgkin`s lymphoma (A) and EBV infectious mononucleosis (B) stained with LMP-1 (Epstein Barr virus), cAb (D24-G) (Prod. No. ALX-810-231).
Kindly performed and provided by Prof. Dr. Med. Lukás Plank, Ph. D. from Department of Pathology and National Consultation Center for Haematopathology of Comenius University, Jessenius Medical Faculty and Faculty Hospital Martin, Slovak Republic.
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810-231

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