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S100 (human) clonal antibody (D28-E)

 
ALX-810-220-R500 500 µl 552.00 USD
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Product Specification

Clone:D28-E
 
Host:Rabbit
 
Isotype:IgG
 
Immunogen:Synthetic peptide corresponding to a C-terminal sequence of human S100-A1 protein.
 
Species reactivity:Human
 
Applications:IHC (FS), IHC (PS)
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:100-1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
 
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
 
Handling:After opening, prepare aliquots and store at +4°C.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
 
Protocol:IHC (paraffin sections) Protocol:
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each
2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each
3. Rinse in distilled water
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes
5. Wash in distilled water for 5 minutes
6. Wash in 0.05M TRIS/HCl, pH 7.6, supplemented with 1% of Tween-20 (buffer A) for 5 minutes
7. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100-1:200 for 1 hour in the closed wet chamber
8. Wash twice 5 minutes with buffer A
9. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB)
10. Wash twice 5 minutes with buffer A
11. Apply the chromogen (DAB), 10 minutes
12. Wash in water for 10 minutes
13. Stain in hematoxylin for 5 minutes
14. Wash in water for 10 minutes
15. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each
16. Wash the section in 2 changes of xylene for 2 minutes each
17. Mount the slide for observation
 
810-220
Figure: Formalin-fixed and paraffin-embedded human neurofibroma tissue (left; 4μm), skin melanoma tissue (middle; 4μm), and human breast tissue (right; 4μm) stained with cAb to S100 (human) (Prod. No. ALX-810-220) show: Left - positive immunostaining of tumor cells and peripheral nerve; Middle - strong positive immunostaining of melanoma cells; Right - strong positive immunostaining of the outer myoepithelial cell component in mammary lobule. Kindly performed and provided by Katarína Poliaková, MD and Lubomír Straka, MD, Ph.D. from Clinical Pathology Presov, Ltd., Presov, Slovakia.
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810-220

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