Actin (human), cAb (S12-I) - Enzo Life Sciences

ALX-810-201

Revised 01-Feb-10

Actin (human), cAb (S12-I)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Actin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-810-201-R100		100 µl	160.00 USD
ALX-810-201-R500		500 µl	317.00 USD
ALX-810-201-L001		1 ml	528.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	S12-I
ISOTYPE:	Rabbit IgG
PURITY DETAIL:	Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
FORMULATION:	Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to a C-terminal sequence of human β-actin.
SPECIFICITY:	Recognizes human actin.
APPLICATION:	Immunohistochemistry (frozen sections, paraffin sections (1:100-1:500)) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 18 months after receipt when stored as recommended.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

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General Information

BACKGROUND/TECHNICAL INFORMATION

Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.

IHC (paraffin sections) Protocol:
1. Deparaffinize the section in 3 changes of xylene, 5 minutes each
2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each
3. Rinse in distilled water
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H₂O₂) for 10 minutes
5. Wash in distilled water for 5 minutes
6. Wash in 0.05M TRIS/HCl, pH 7.6, supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes
7. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100 for 1 hour in the closed wet chamber
8. Wash twice 5 minutes with buffer A
9. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB)
10. Wash twice 5 minutes with buffer A
11. Apply the chromogen (DAB), 10 minutes
12. Wash in water for 10 minutes
13. Stain in hematoxylin for 5 minutes
14. Wash in water for 10 minutes
15. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each
16. Wash the section in 2 changes of xylene for 2 minutes each
17. Mount the slide for observation

Further Categories Containing This Product:

Monoclonal Antibodies