Actin (human), cAb (S12-I)
| ALX-810-201-R100 | 100 µl | 160.00 USD |  |
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| ALX-810-201-R500 | 500 µl | 317.00 USD | |
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| ALX-810-201-L001 | 1 ml | 528.00 USD | |
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Product Specification
| Purity Detail: | Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide. |
| Formulation: | Liquid. In 20mM TRIS/HCl, pH 8.0, containing 20mg/ml BSA and 0.05% sodium azide. |
| Clone: | S12-I |
| Isotype: | Rabbit IgG |
| Immunogen: | Synthetic peptide corresponding to a C-terminal sequence of human β-actin. |
| Specificity: | Recognizes human actin. |
| Application: | Immunohistochemistry (frozen sections, paraffin sections (1:100-1:500)) Optimal conditions must be determined individually for each application. |
| Shipping: | SHIPPED ON BLUE ICE |
| Long Term Storage: | -20°C |
| Use/Stability: | Stable for at least 18 months after receipt when stored as recommended. |
| Handling: | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Background / Technical Information: | Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity. IHC (paraffin sections) Protocol: 1. Deparaffinize the section in 3 changes of xylene, 5 minutes each 2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each 3. Rinse in distilled water 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes 5. Wash in distilled water for 5 minutes 6. Wash in 0.05M TRIS/HCl, pH 7.6, supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes 7. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100 for 1 hour in the closed wet chamber 8. Wash twice 5 minutes with buffer A 9. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB) 10. Wash twice 5 minutes with buffer A 11. Apply the chromogen (DAB), 10 minutes 12. Wash in water for 10 minutes 13. Stain in hematoxylin for 5 minutes 14. Wash in water for 10 minutes 15. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each 16. Wash the section in 2 changes of xylene for 2 minutes each 17. Mount the slide for observation |
Figure: Formalin-fixed and paraffin-embedded human stomach tissue (4μm) stained with cAb to actin (Prod. No. ALX-810-201), shows strong positive immunostaining of smooth muscle cells.Kindly performed and provided by Katarína Poliaková, MD and Lubomír Straka, MD, Ph.D. from Clinical Pathology Presov, Ltd., Presov, Slovakia.
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