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Nephrin (Ig-like domain 5) clonal antibody (Y17-R)

 
ALX-810-016-R100 100 µl 486.00 USD
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Product Specification

Alternative Name:Renal glomerulus-specific cell adhesion receptor
 
Clone:Y17-R
 
Host:Rabbit
 
Isotype:IgG
 
Immunogen:Synthetic peptide corresponding to an epitope in the centre of the fifth Ig-like domain of human nephrin (between aa 465 and 528).
 
UniProt ID:O60500
 
Species reactivity:Human, Mouse, Rat
 
Applications:ELISA, IHC (PS), WB
 
Recommended Dilutions/Conditions:ELISA (1:50,000-1:100,000)
Immunohistochemistry (paraffin sections, 1:200)
Western Blot (1:5,000; Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100, Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
 
Formulation:Liquid. In 20mM TRIS/HCl, pH 8.0, containing 10mg/ml BSA and 0.05% sodium azide.
 
Use/Stability:Stable for at least 12 months after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.
 

Product Literature References

The Dose-Dependent Organ-Specific Effects of a Dipeptidyl Peptidase-4 Inhibitor on Cardiovascular Complications in a Model of Type 2 Diabetes: J.Y. Moon, et al.; PLoS One 11, e0150745 (2016), Application(s): Immunohistochemistry, Abstract; Full Text

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