Actin, cAb (S12-I)



ALX-810-001-R100	100 µl	352.00 USD

Product Specification

Purity Detail:	Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.

Formulation:	Liquid. In 20mM TRIS/HCl, pH 8.0, containing 10mg/ml BSA and 0.05% sodium azide.

Clone:	S12-I

Isotype:	Rabbit IgG

Immunogen:	Synthetic peptide corresponding to a C-terminal sequence of human β-actin.

Specificity:	Recognizes human, mouse and rat α-actin and β-actin.

Application:	ELISA (1:10’000-1:20’000) Immunohistochemistry (frozen sections; paraffin sections) Immunoprecipitation Western Blot (1:2’000) Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100 Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Optimal conditions must be determined individually for each application.

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 18 months after receipt when stored as recommended.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by "in vitro cloning technology". The resulting product is monospecific and characterized by superior specificity, affinity and avidity.