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MHC Class I monoclonal antibody (W6/32)

 
ALX-805-711-C100 100 µg 345.00 USD
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Product Specification

Alternative Name:HLA Class I
 
Clone:W6/32
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Membrane of human tonsil cells.
 
Species reactivity:Human
Bovine, Cat, Monkey
 
Specificity:Recognizes virtually all nucleated human cells. Suitable as positive control for HLA tissue typing and crossmatching.
 
Crossreactivity:Does not cross-react with rabbit MHC class I.
 
Applications:ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB, FUNC
 
Recommended Dilutions/Conditions:Western Blot (non-reducing)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.4. Sterile filtered. Contains no preservatives.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Scientific Background:HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
 

Product Literature References

Expression of HLA-G in human cornea, an immune-privileged tissue: M. Le Discorde, et al.; Hum. Immunol. 64, 1039 (2003), Abstract;
HLA-B27 misfolding is associated with aberrant intermolecular disulfide bond formation (dimerization) in the endoplasmic reticulum: N.S. Dangoria, et al.; J. Biol. Chem. 277, 23459 (2002), Abstract; Full Text
The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex: T.M. Tran, et al.; Immunogenetics 53, 440 (2001), Abstract;
Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody: J.J. Ladasky, et al.; Immunogenetics 49, 312 (1999), Abstract;
Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin: M.J. Shields and R.K. Ribaudo; Tissue Antigens 51, 567 (1998), Abstract;
Major histocompatibility complex class I molecule expression is normal on peripheral blood lymphocytes from patients with insulin-dependent diabetes mellitus: W. Hao, et al.; J. Clin. Invest. 98, 1613 (1996), Abstract; Full Text
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species: C.N. Jacobsen, et al.; Vet. Immunol. Immunopathol. 39, 461 (1993), Abstract;
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast: P.L. Stern, et al.; J. Immunol. 138, 1088 (1987), Abstract;
Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin: F. Kievits and P. Ivanyi; Hum. Immunol. 50, 115 (1987), Abstract;
A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes: J.J. Neefjes, et al.; Immunogenetics 23, 341 (1986), Abstract;
Quantitative analysis of platelet surface HLA by W6/32 anti-HLA monoclonal antibody: K.J. Kao, et al.; Blood 68, 627 (1986), Abstract; Full Text
Human chromosome 11 carries at least four genes controlling expression of cell-surface antigens: A. Tunnacliffe, et al.; Somatic Cell Genet. 9, 629 (1983), Abstract;
Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies: F.M. Brodsky and P. Parham; Immunogenetics 15, 151 (1982), Abstract;
The gene, MIC4, which controls expression of the antigen defined by monoclonal antibody F10.44.2, is on human chromosome 11: P.N. Goodfellow, et al.; Eur. J. Immunol. 12, 659 (1982), Abstract;
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis: C.J. Barnstable, et al.; Cell 14, 9 (1978), Abstract;

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