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HLA-G (human) monoclonal antibody (MEM-G/1)

ALX-805-704-C100 100 µg 296.00 USD
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Product Specification

Immunogen:Human recombinant HLA-G denatured heavy chain.
UniProt ID:P17693
Species reactivity:Human
Specificity:Recognizes denatured HLA-G heavy chain.
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, stain by standard ABC technique, pretraet by heat retrieval in 0.01 citrate buffer (4 x 2 minutes in microwave oven); recommended starting dilution is 1:60-1:100; incubate 1 hour at room temperature; positive tissue: Human placenta - extravillous cytotrophoblast)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein A affinity chromatography.
Formulation:Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Figure: Immunohistochemistry analysis (AEC visualization) of paraffin embedded section of human lung carcinoma using Prod. No. ALX-805-704.
The antibody MEM-G/1 detects HLA-G molecules in activated macrophages infiltrating lung carcinomas.
The expression of HLA-G in myelo-monocytic cells infiltrating lung pathologic tissues could alter antigenic presentation and contribute to decrease immune response efficiency, subsequently favoring the progression of tumoral or inflammatory process.
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Product Literature References

A critical look at HLA-G: R. Apps, et al.; Trends Immunol. 29, 313 (2008), Abstract;
Disulfide bond-mediated dimerization of HLA-G on the cell surface: J.E. Boyson, et al.; PNAS 99, 16180 (2002), Abstract;
Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules: S. Fournel, et al.; Tissue Antigens 55, 510 (2000), Abstract;
Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment: G. Frumento, et al.; Tissue Antigens 56, 30 (2000), Abstract;

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