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HLA-E (human) monoclonal antibody (MEM-E/02)

 
ALX-805-701-C100 100 µg 237.00 USD
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Product Specification

Clone:MEM-E/02
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human HLA-E denatured heavy chain.
 
UniProt ID:P13747
 
Species reactivity:Human
 
Specificity:Recognizes denatured heavy chain of human HLA-E. Specificity was confirmed on HLA-G/HLA-E Workshop (Victoria 2002).
 
Crossreactivity:Does not cross-react with HLA-A, -B, -C or -G.
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, recommended starting dilution is 5-10µg/ml; heat antigen retrieval (sodium citrate); incubate 1 hour at room temperature; detection DAB)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
 
Use/Stability:Stable for at least 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

Therapeutic induction of regulatory, cytotoxic CD8+ T cells in multiple sclerosis: D.K. Tennakoon, et al.; J. Immunol. 176, 7119 (2006), Abstract;
Identification of a human HLA-E-restricted CD8+ T cell subset in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine: R. Salerno-Goncalves, et al.; J. Immunol. 173, 5852 (2004), Abstract; Full Text
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules: C. Menier, et al.; Hum. Immunol. 64, 315 (2003), Abstract;

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