Recombinant human HLA-G refolded with β2-microglobulin and peptide.
Recognizes native HLA-G on the cell surface as well as soluble HLA-G molecule in its β2-microglobulin associated form.
ELISA, Flow Cytometry, ICC, IHC (FS), IP
ELISA (capture; in combination with Prod. No. ALX-804-482) Flow Cytometry (1-5µg/ml; JEG-3 cells could be used as positive control) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purified by precipitation.
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
Blue Ice Not Frozen
Short Term Storage:
Long Term Storage:
Product Literature References
A critical look at HLA-G: R. Apps, et al.; Trends Immunol. 29, 313 (2008), Abstract;
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules: C. Menier, et al.; Hum. Immunol. 64, 315 (2003), Abstract;
HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy: B. Fuzzi, et al.; Eur. J. Immunol. 32, 311 (2002), Abstract;
Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases: C. Pangault, et al.; Hum. Immunol. 63, 83 (2002), Abstract;
Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule: J.M. Lozano, et al.; Aids 16, 347 (2002), Abstract;
Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules: S. Fournel, et al.; Tissue Antigens 55, 510 (2000), Abstract;