CEACAM20 (human), mAb (HT-12D8)

Figure 2: Specificity testing by flow cytometry.Method: FACS analysis of BOSC23 cells using CEACAM20 (human), mAb (HT-12D8) (Prod. No. ALX-805-090). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM20 (red curve) or an irrelevant protein (control transfectant). Binding of CEACAM20 (human), mAb (HT-12D8) was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM20 transfected cells.

Figure 3: Specificity testing by spectral confocal microscopy.Method: Spectral confocal microscopy of CHO cells using CEACAM20 (human), mAb (HT-12D8) (Prod. No. ALX-805-090). CHO cells were transiently transfected with an expression vector encoding CEACAM20. Binding of CEACAM20 (human), mAb (HT-12D8) was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

Figure 4: Antibody cross-reactivity with members of the CEA family.Method: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, CEACAM20 (human), mAb (HT-12D8) (Prod. No. ALX-805-090) was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM20 transfected cells (red curve).