United States
CEACAM8 (human), mAb (GM2H6)
| ALX-805-088-C100 | 100 µg | 464.00 USD |  |
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Product Specification
| Alternative Name: | Carcinoembryonic antigen-related cell adhesion molecule 8, CD66b |
| Concentration: | 1mg/ml |
| Purity Detail: | Protein G-affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2. Contains no preservatives. |
| Clone: | GM2H6 |
| Isotype: | Mouse IgG1 |
| Immunogen: | Vector containing the cDNA of human CEACAM8. |
| Quality Control: | Specificity routinely tested by flow cytometry on CEACAM8 transfected BOSC23 cells. |
| Specificity: | Recognizes human CEACAM8. Does not cross-react with human CEACAM1, 3, 4, 5, 6, 7, or PSG1. |
| Application: | Competitive ELISA (1.2µg/106 cells) Flow Cytometry (1:200-1:400) Optimal conditions must be determined individually for each application. |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Handling: | Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Background / Technical Information: | CEA-related cell adhesion molecule 8 (CEACAM8) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a molecular mass of of 95kD and is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. Therefore CEACAM8 could serve as a marker for granulocyte activities. Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A and B domains. |
Figure 1: Specificity testing by flow cytometry.Method: FACS analysis of BOSC23 cells using CEACAM8 (human), mAb (GM2H6) (Prod. No. ALX- 805-088). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of CEACAM8 (human), mAb (GM2H6) was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.
Figure 2: Antibody cross-reactivity with members of the CEA family.Method: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (green curves). An irrelevant monoclonal antibody served asa negative control (black curves). For specificity testing, CEACAM8 (human), mAb (GM2H6) (Prod. No. ALX- 805-088) was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).
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General Literature References
Carcinoembryonic antigen: W. Zimmermann; Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York 459 (2002),
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues: S. Hammarström; Semin. Cancer Biol. 9, 67 (1999), (Review), Abstract;
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