CEACAM5,6 (human), mAb (MUS)



ALX-805-086-C100	100 µg	464.00 USD

Product Specification

Alternative Name:	Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen-related cell adhesion molecule 6, CD66e, CD66c

Concentration:	1mg/ml

Purity Detail:	Protein G-affinity purified.

Formulation:	Liquid. In PBS, pH 7.2. Contains no preservatives.

Clone:	MUS

Isotype:	Mouse IgG1

Immunogen:	Extracted human CEACAM5.

Quality Control:	Specificity routinely tested by flow cytometry on CEACAM5 and CEACAM6 transfected BOSC23 cells.

Specificity:	Recognizes human CEACAM5 and 6. Does not cross-react with human CEACAM1, 3, 4, 7 or 8.

Application:	Flow Cytometry (1.2µg/10⁶ cells) ELISA (1:200-1:400) Competitive ELISA (1:200) Immunohistochemistry (frozen sections) (1-2µg/10⁶ cells) Western Blot (4µg/ml) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Background / Technical Information:	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM6 expression is strongly up-regulated already during early stages of adenocarcinoma formation. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Figure 1: Specificity testing by flow cytometry.Method: FACS analysis of BOSC23 cells using CEACAM5,6 (human), mAb (MUS) (Prod. No. ALX-805-086). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of CEACAM5,6 (human), mAb (MUS) was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM5 transfected cells.

Figure 2: Specificity testing by flow cytometry.Method: FACS analysis of BOSC23 cells using CEACAM5,6 (human), mAb (MUS) (Prod. No. ALX-805-086). BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of CEACAM5,6 (human), mAb (MUS) was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM6 transfected cells.

Figure 3: Antibody cross-reactivity with members of the CEA family.Method: BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, CEACAM5,6 (human), mAb (MUS) (Prod. No. ALX-805-086) was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM5 and CEACAM6 expressing cells (red curves).

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Product Literature References

Carcinoembryonic antigen: W. Zimmermann; Wiley Encyclopedia of Molecular Medicine (2002), John Wiley & Sons Inc., New York, USA, pp. 459,

Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas: S. Scholzel, et al.; Am. J. Pathol. 156, 595 (2000), Abstract; Full Text

CEA-related proteins on human urothelial cell lines of different transformation grades: A. Krop-Watorek, et al.; Cancer Lett. 139, 15 (1999), Abstract;

Opa binding to cellular CD66 receptors mediates the transcellular traversal of Neisseria gonorrhoeae across polarized T84 epithelial cell monolayers: J. Wang, et al.; Mol. Microbiol. 30, 657 (1998), Abstract; Full Text

CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules: F. Grunert; Leukocyte Typing VI: White cell Differentiation Antigens (1996), Garland Publishing Inc., New York and London, pp. 1012,

Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin: H. Hanenberg; Leukemia 12, 2127 (1994),

A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members: J. Thompson, et al.; Int. J. Cancer 55, 311 (1993), Abstract;

Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants: S. Daniel, et al.; Int. J. Cancer 55, 303 (1993), Abstract;

General Literature References

The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues: S. Hammarstrom; Semin. Cancer Biol. 9, 67 (1999), (Review), Abstract;

Carcinoembryonic antigen gene family: molecular biology and clinical perspectives: J.A. Thompson, et al.; J. Clin. Lab. Anal. 5, 344 (1991), (Review), Abstract;