CEACAM6 (human), mAb (9A6)



ALX-805-083-C100	100 µg	463.00 USD

Product Specification

Alternative Name:	CD66c, NCA

Concentration:	1.8mg/ml

Purity Detail:	Protein G-affinity purified.

Formulation:	Liquid. In PBS, pH 7.2. Contains no preservatives.

Clone:	9A6

Isotype:	Mouse IgG1

Immunogen:	Tumor cell lines expressing human CEACAM6.

Quality Control:	Routinely tested by flow cytometry on BOSC cells transiently transfected with a NCA expression vector.

Specificity:	Recognizes human CEACAM6. Does not cross-react with human CEACAM1, 3, 4, 5, 7 or 8.

Application:	Flow Cytometry ELISA Immunohistochemistry (frozen sections) Western Blot Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Miscellaneous/General:	The normal cross-reacting antigen (NCA) or CEACAM6 belongs to the carcinoembryonic antigen (CEA) gene family. CEACAM6 is a GPI-anchored membrane protein with a single N domain, which shows structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like domains.

Figure: Reactivity of FITC-labelled MAb to CEACAM6 (human) (9A6) (Prod. No. ALX-805-083) with polymorph nuclear cells. 2x10⁵ polymorph nuclear cells (PMN) with (green) or without (blue) prior incubation with PMA were reacted for 30 min. at 4°C with FITC conjugated MAb to CEACAM6 (human) (9A6) (40µg/ml) or with an isotype matched FITC-conjugated irrelevant MAb (red), then washed twice. PMN expression of CEACAM6 is constitutively and can be augmented by treatment with PMA.

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Product Literature References

Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens: T. Schmitter, et al.; J. Exp. Med. 199, 35 (2004), Abstract; Full Text

Carcinoembryonic antigen: W. Zimmermann; Wiley Encyclopedia of Molecular Medicine (2002), John Wiley & Sons Inc., New York, USA, pp. 459,

Carcinoembryonic antigen-related cell adhesion molecule 1 expression and signaling in human, mouse, and rat leukocytes: evidence for replacement of the short cytoplasmic domain isoform by glycosylphosphatidylinositol-linked proteins in human leukocytes: B.B. Singer, et al.; J. Immunol. 168, 5139 (2002), Abstract; Full Text

Nuclear factor-kappa B directs carcinoembryonic antigen-related cellular adhesion molecule 1 receptor expression in Neisseria gonorrhoeae-infected epithelial cells: P. Muenzner, et al.; J. Biol. Chem. 277, 7438 (2002), Abstract; Full Text

Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site: S.M. Watt, et al.; Blood 98, 1469 (2001), Abstract; Full Text

Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1; previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto: P. Muenzner, et al.; J. Biol. Chem. 276, 24331 (2001), Abstract; Full Text

Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas: S. Scholzel, et al.; Am. J. Pathol. 156, 595 (2000), Abstract; Full Text

Carcinoembryonic antigen family receptor specificity of Neisseria meningitidis Opa variants influences adherence to and invasion of proinflammatory cytokine-activated endothelial cells: P. Muenzner, et al.; Infect. Immun. 68, 3601 (2000), Abstract; Full Text

CEA-related proteins on human urothelial cell lines of different transformation grades: A. Krop-Watorek, et al.; Cancer Lett. 139, 15 (1999), Abstract;

Opa binding to cellular CD66 receptors mediates the transcellular traversal of Neisseria gonorrhoeae across polarized T84 epithelial cell monolayers: J. Wang, et al.; Mol. Microbiol. 30, 657 (1998), Abstract; Full Text

CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules: F. Grunert; Leukocyte Typing VI: White cell Differentiation Antigens (1996), Garland Publishing Inc., New York and London, pp. 1012,

Expression of the CEA gene family members NCA-50/90 and NCA-160 (CD66) in childhood acute lymphoblastic leukemias (ALLs) and in cell lines of B-cell origin: H. Hanenberg; Leukemia 12, 2127 (1994),

A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members: J. Thompson, et al.; Int. J. Cancer 55, 311 (1993), Abstract;

Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants: S. Daniel, et al.; Int. J. Cancer 55, 303 (1993), Abstract;

Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia: H.G. Gottlinger, et al.; Cancer Res. 48, 2198 (1988), Abstract;

General Literature References

The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues: S. Hammarstrom; Semin. Cancer Biol. 9, 67 (1999), (Review), Abstract;

Carcinoembryonic antigen gene family: molecular biology and clinical perspectives: J.A. Thompson, et al.; J. Clin. Lab. Anal. 5, 344 (1991), (Review), Abstract;