pan-CEACAM (1,5,6,8) (human), mAb (TET2)
[CD66a/CD66b/CD66c/CD66e, BGP, CEA, NCA and CGM6]
| ALX-805-081-C100 | 100 µg | 450.00 USD |  |
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Product Specification
| Concentration: | 1mg/ml |
| Purity Detail: | Protein G-affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 0.01% sodium azide. |
| Clone: | TET2 |
| Isotype: | Mouse IgG2b |
| Immunogen: | CEACAM5 (CEA) partially purified from a perchloric acid extract from liver metastases of colonic tumors. |
| Quality Control: | Routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector. |
| Specificity: | Recognizes human CEACAM1, 5, 6 and 8. |
| Application: | ELISA Flow Cytometry Immunohistochemistry (frozen sections) Western Blot. Optimal conditions must be determined individually for each application. |
| Shipping: | SHIPPED ON BLUE ICE |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Handling: | Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Miscellaneous/General: | CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leukocytes. Overexpression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocytes and dendritic cell activation, receptors of Neisseria species and other bacteria. |
Figure 1: Specificity testing of TET2 (Prod. No. ALX-805-081). BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11 (Prod. No. ALX-805-080); CEACAM7: CAC2; CEACAM8: 80H3; PSG1: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified TET2 was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM1, CEACAM5, CEACAM6 and CEACAM8 expressing cells (red curves).
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Product Literature References
Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns: F. Grunert, et al.; Int. J. Cancer 36, 357 (1985), Abstract;
CD66 family Workshop: Binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules: F. Grunert, et al.; Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto, et al., eds.), Garland Publishing Inc., New York 1012 (1996),
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site: S.M. Watt, et al.; Blood 98, 1469 (2001), Abstract; Full Text
General Literature References
Carcinoembryonic antigen gene family: molecular biology and clinical perspectives J.A. Thompson, et al. J. Clin. Lab. Anal. 5 344 (1991) (Review) Abstract
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues S. Hammarström Semin. Cancer Biol. 9 67 (1999) (Review) Abstract
Carcinoembryonic antigen W. Zimmermann Wiley Encylopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York 459 (2002)
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