CD66e (human), mAb (26/3/13)
[CEACAM5, CEA, Carcinoembryonic antigen]
| ALX-805-077-C100 | 100 µg | 450.00 USD |  |
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Product Specification
| Concentration: | 1mg/ml |
| Purity Detail: | Protein G-affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 0.01% sodium azide. |
| Clone: | 26/3/13 |
| Isotype: | Mouse IgG1 |
| Immunogen: | CD66e (CEACAM5; CEA) partially purified from a perchloric acid extract from liver metastases of colonic tumors. |
| Quality Control: | Routinely tested by flow cytometry on BOSC cells transiently transfected with a CD66e expression vector. |
| Specificity: | Recognizes human CD66e. Does not cross-react with human CEACAM1, 3, 4, 6, 7, 8 or PSG. |
| Application: | ELISA Flow Cytometry Immunohistochemistry (frozen sections) Western Blot Optimal conditions must be determined individually for each application. |
| Shipping: | SHIPPED ON BLUE ICE |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Handling: | Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Miscellaneous/General: | CD66e (CEACAM5; CEA) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 180'000-200'000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cells of the tongue, esophagus and cervix. CD66e is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). |
Figure 1: Specificity testing of 26/3/13 (Prod. No. ALX-805-077). Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7 or 8. Recognition of CEACAM3, 4 and of a recombinant transmembrane-anchor-ed PSG1 fusion protein was tested on stably transfected HeLa (CEACAM3, PSG) and CHO cells (CEACAM4), respectively. Expression of the constructs was confirmed with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11 (Prod. No. ALX-805-080); CEACAM7: CAC2; CEACAM8: TET2 (Prod. No. ALX-805-081); PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified 26/3/13 was tested on all CEACAM transfectants. A positive signal was only obtained with CEACAM5 expressing cells (red curves).
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Product Literature References
Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns: F. Grunert, et al.; Int. J. Cancer 36, 357 (1985), Abstract;
Effects of human bone marrow stroma on the growth of human tumor cells: E.S. Strobel, et al.; Cancer Res. 49, 1001 (1989), Abstract;
A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members: J. Thompson, et al.; Int. J. Cancer 55, 311 (1993), Abstract;
CD66 family workshop: binding of myeloid blind panel antibodies and CD66 Subsection antibodies to HeLa transfectants expressing individual CD66 molecules: F. Grunert, et al.; Leukocyte Typing VI: White cell Differentiation Antigens (T. Kishimoto, et al., eds.), Garland Publishing Inc., New York and London 1012 (1996),
CEA-related proteins on human urothelial cell lines of different transformation grades: A. Krop-Watorek, et al.; Cancer Lett. 139, 15 (1999), Abstract;
Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas: S. Scholzel, et al.; Am. J. Pathol. 156, 595 (2000), Abstract; Full Text
General Literature References
Carcinoembryonic antigen gene family: molecular biology and clinical perspectives J.A. Thompson, et al. J. Clin. Lab. Anal. 5 344 (1991) (Review) Abstract
The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues S. Hammarström Semin. Cancer Biol. 9 67 (1999) (Review) Abstract
Carcinoembryonic antigen W. Zimmermann Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York 459 (2002)
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