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CD45 (human) monoclonal antibody (MEM-28)

 
ALX-805-041-C100 100 µg 194.00 USD
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Product Specification

Clone:MEM-28
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Human thymocytes and T lymphocytes.
 
UniProt ID:P08575
 
Species reactivity:Human
 
Specificity:Recognizes all isoforms of CD45.
 
Applications:Flow Cytometry, ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (1µg/ml)
Immunocytochemistry (10µg/ml, paraformaldehyde fixation can be used)
Immunohistochemistry (paraffin sections, no pre-treatment of tissue sections is essential)
Western Blot (1µg/ml; Positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2x non-reducing SDS.
Application note: non-reducing conditions. SDS-PAGE (6% separating gel))
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 

Product Literature References

Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules: L. Cermak, et al.; J. Biol. Chem. 277, 7955 (2002), Abstract; Full Text
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74): V. Horejsi, et al.; Folia Biol (Praha) 34, 23 (1988), Abstract;
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen: V. Horejsi, et al.; Folia Biol (Praha) 32, 12 (1986), Abstract;

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