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Fas (human) monoclonal antibody (2R2)

 
ALX-805-038-C100 100 µg 419.00 USD
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Product Specification

Alternative Name:CD95, APO-1, TNFRSF 6
 
Clone:2R2
 
Host:Mouse
 
Isotype:IgG3
 
Immunogen:Mouse L cells stably overexpressing human Fas (CD95; APO-1).
 
UniProt ID:P25445
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Applications:Flow Cytometry, FUNC
 
Recommended Dilutions/Conditions:Flow Cytometry, Functional Application (induces apoptosis - no cross-linking required). , - Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation., Recommended working dilutions are 1:200-1:1,000.
 
Application Notes:Flow Cytometry
Functional Application (induces apoptosis - no cross-linking required).
- Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation.
Recommended working dilutions are 1:200-1:1000.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS containing 1% BSA. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Fas (human) monoclonal antibody (2R2) Immunofluorescence
Figure: Detection of Fas surface expression by FACS analysis.
Method: Normal L cells (open histogram) and cells stably overexpressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunstaining was obtained with an isotype matched IgG control.
Fas (human) monoclonal antibody (2R2) Flow Cytometry
Figure: Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells.
Method: (A) Dose response: Jurkat cells (1 x 105) were treated with agonistic anti-Fas 2R2 antibodies at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry.
(B) Time course of apoptosis: Jurkat cells (1 x 105) were treated with 1 µg/ml anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA.
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Fas (human) monoclonal antibody (2R2) Immunofluorescence Fas (human) monoclonal antibody (2R2) Flow Cytometry

Product Literature References

IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000), Abstract;
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999), Abstract; Full Text
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998), Abstract; Full Text

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