Product Details
| Alternative Name: | CASPER, CLARP, I-FLICE, Usurpin, CFLAR |
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| Clone: | 7F10 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human FLIP. |
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| UniProt ID: | O15519 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
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| Applications: | ELISA, Flow Cytometry, IHC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with the adapter protein, FADD, and caspase-8 and –10, and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R and TNFR1. |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of FLIP (human), mAb (7F10) (Prod. No. ALX-804-961): Lane 1: MW marker, Lane 2&4: HeLa cell lysate, Lane 3&5: Jurkat cell lysate. The two membranes were blotted with 7F10 or NF6 clones at 2µg/ml separately. The result showed they detected very similar pattern in these cell lysates.
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Product Literature References
DR5 Up-Regulation Induced by Dichloroacetate Sensitizes Tumor Cells to Lipid Nanoparticles Decorated with TRAIL: J.M. Brualla, et al.; J. Clin. Med.
12, 608 (2023),
Abstract;
Exploring the MEN1 dependent modulation of caspase 8 and caspase 3 in human pancreatic and murine embryo fibroblast cells: N. Wagener, et al.; Apoptosis
27, 70 (2022),
Abstract;
Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5: S.M. Woo, et al.; Cell Death Dis.
13, 821 (2022),
Abstract;
Lucanthone, Autophagy Inhibitor, Enhances the Apoptotic Effects of TRAIL through miR-216a-5p-Mediated DR5 Upregulation and DUB3-Mediated Mcl-1 Downregulation: J.Y. Yoon, et al.; Int. J. Mol. Sci.
23, 17 (2022),
Abstract;
PKR and TLR3 trigger distinct signals that coordinate the induction of antiviral apoptosis: W. Zuo, et al.; Cell Death Dis.
13, 707 (2022),
Abstract;
Potent pro-apoptotic combination therapy is highly effective in a broad range of cancers: A. Montinaro, et al.; Cell Death Differ.
10, 1038 (2021),
Abstract;
Selectively targeting myeloid-derived suppressor cells through TRAIL receptor 2 to enhance the efficacy of CAR T cell therapy for treatment of breast cancer: S.A. Nalawade, et al.; J. Immunother. Cancer
9, 3237 (2021),
Abstract;
3-O-acetylrubianol C (3AR-C) induces RIPK1-dependent programmed cell death by selective inhibition of IKKβ: K. Kang, et al.; FASEB J.
34, 4369 (2020),
Abstract;
Co-treatment of birinapant with TRAIL synergistically induces apoptosis by downregulating cFLIP(L) in MDA-MB-453 cell lines: E.J. Park, et al.; Biochem. Biophys. Res. Commun.
533, 289 (2020),
Abstract;
Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells: A. Wentao, et al.; J. Exp. Clin. Cancer Res.
38, 181 (2019),
Abstract;
Full Text
Involvement of up-regulation of death receptors and Bim in Hispolon-mediated TNF-related apoptosis-inducing ligand sensitization in human renal carcinoma: J.M. Yun, et al.; J. Cancer Prev.
24, 155 (2019),
Application(s): Western-blot using Caki cells,
Abstract;
Full Text
The arginine methyltransferase PRMT5 and PRMT1 distinctly regulate the degradation of anti-apoptotic protein CFLARL in human lung cancer cells: M. Li, et al.; J. Exp. Clin. Cancer Res.
38, 64 (2019),
Abstract;
Full Text
The transcription factor SP3 drives TNF-α expression in response to Smac mimetics: S.T. Beug, et al.; Sci. Signal.
12, eaat9563 (2019),
Abstract;
Acquired Resistance of ER-Positive Breast Cancer to Endocrine Treatment Confers an Adaptive Sensitivity to TRAIL through Posttranslational Downregulation of c-FLIP: L. Piggott, et al.; Clin. Cancer Res.
24, 2452 (2018),
Abstract;
Differential expression patterns of the short and long isoform of cFLIP on FasL-mediated apoptosis: H.J. Um, et al.; Oncol. Rep.
39, 2443 (2018),
Abstract;
Differential expression patterns of the short and long isoform of cFLIP on FasL‑mediated apoptosis: H.J. Um, et al.; Oncol. Rep.
39, 2443 (2018),
Abstract;
Loss of functional BAP1 augments sensitivity to TRAIL in cancer cells: K.K. Kolluri, et al.; Elife
7, e30224 (2018),
Abstract;
Full Text
Metformin-induced apoptosis facilitates degradation of the cellular caspase 8 (FLICE)-like inhibitory protein through a caspase-dependent pathway in human renal cell carcinoma A498 cells: J.H. Jang, et al.; Oncol. Lett.
16, 2030 (2018),
Abstract;
Full Text
TRAIL-Induced Apoptosis in TRAIL-Resistant Breast Carcinoma Through Quercetin Cotreatment: J.M. Manouchehri, et al.; Breast Cancer (Auckl.)
12, 1178223417749855 (2018),
Application(s): Western blot,
Abstract;
Full Text
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation: C.M. Henry, et al.; Mol. Cell
65, 715 (2017),
Abstract;
cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition: C. Padmanabhan, et al.; Oncotarget
8, 101072 (2017),
Abstract;
Full Text
Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways: M.C. Tanzer, et al.; Cell Death Differ.
24, 481 (2017),
Abstract;
Full Text
Role of p63 and p73 isoforms on the cell death in patients with hepatocellular carcinoma submitted to orthotopic liver transplantation: R. Gonzalez, et al.; PLoS One
12, e0174326 (2017),
Abstract;
Full Text
Autophagy-Mediated Degradation of IAPs and c-FLIP(L) Potentiates Apoptosis Induced by Combination of TRAIL and Chal-24: J. Xu, et al.; J. Cell. Biochem.
117, 1136 (2016),
Abstract;
Full Text
Galangin sensitizes TRAIL-induced apoptosis through down-regulation of anti-apoptotic proteins in renal carcinoma Caki cells: M.A. Han, et al.; Sci. Rep.
6, 18642 (2016),
Abstract;
Full Text
Infection of epithelial cells with Chlamydia trachomatis inhibits TNF-induced apoptosis at the level of receptor internalization while leaving non-apoptotic TNF-signalling intact: C. Waguia Kontchou, et al.; Cell. Microbiol.
18, 1583 (2016),
Abstract;
Overcoming resistance to TRAIL-induced apoptosis in solid tumor cells by simultaneously targeting death receptors, c-FLIP and IAPs: Y. Huang, et al.; Int. J. Oncol.
49, 153 (2016),
Application(s): Western-blot using U2OS cells,
Abstract;
Full Text
Quercetin sensitizes pancreatic cancer cells to TRAIL-induced apoptosis through JNK-mediated cFLIP turnover: J.H. Kim, et al.; Int. J. Biochem. Cell Biol.
78, 327 (2016),
Application(s): Western blot; cell lysates,
Abstract;
Targeting of BCL2 family proteins with ABT-199 and homoharringtonine reveals BCL2- and MCL1-dependent subgroups of diffuse large B-cell lymphoma: M. Klanova, et al.; Clin. Cancer Res.
22, 1138 (2016),
Application(s): Western-blot using BJAB and OCI-Ly7 cells,
Abstract;
YM155 sensitizes TRAIL-induced apoptosis through cathepsin S-dependent down-regulation of Mcl-1 and NF-κB-mediated down-regulation of c-FLIP expression in human renal carcinoma Caki cells: S.M. Woo, et al.; Oncotarget
7, 61520 (2016),
Abstract;
Full Text
6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression: M.A. Han, et al.; Chem. Biol. Interact.
228, 69 (2015),
Abstract;
FAS-ligand regulates differential activation-induced cell death of human T-helper 1 and 17 cells in healthy donors and multiple sclerosis patients: M.T. Cencioni, et al.; Cell. Death Dis.
6, e1741 (2015),
Application(s): Western Blot,
Abstract;
Full Text
G2/M Arrest Sensitises Erythroid Leukemia Cells to TRAIL-induced Apoptosis: S. Cruet-Hennequart, et al.; J. Leuk.
3, 174 (2015),
Application(s): Western Blot,
Full Text
Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis: S. Shi, et al.; Oncotarget
6, 1640 (2015),
Abstract;
Full Text
Helicobacter pylori sensitizes TNF-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in human gastric epithelial cells through regulation of FLIP: W.C. Lin, et al.; Cell Death Dis.
5, e1109 (2014),
Application(s): Western blot using human gastric adenocarcinoma cell line AGS,
Abstract;
Full Text
General Literature References
Cellular FLIP is expressed in cardiomyocytes and down-regulated in TUNEL-positive grafted cardiac tissues: T. Imanishi, et al.; Cardiovasc. Res.
48, 101 (2000),
Abstract;
Full Text
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