Product Details
| Alternative Name: | Helicard, Melanoma differentiation-associated gene 5, Interferon-induced helicase C domain-containing protein 1, RH116, CADM-140 autoantigen, RIG-I-like receptor 2, RLR-2 |
| |
| Clone: | 1B6 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555). |
| |
| UniProt ID: | Q9BYX4 |
| |
| Source: | Purified from hybridoma tissue culture supernatant. |
| |
| Species reactivity: | Human, Mouse
|
| |
| Applications: | ELISA, WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Purity Detail: | Protein G-affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow easier access of CAD, thereby accelerating the degradation of genomic DNA. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Western blot analysis of MDA5 monoclonal antibody (1B6) (Prod. No. ALX-804-878): Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.
Please mouse over
General Literature References
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature
441, 101 (2006),
Abstract;
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS
99, 637 (2002),
Abstract;
Related Products
