IL-12 (p35) (human), mAb (Valy-1)



ALX-804-874-C100	100 µg	360.00 USD

Product Specification

Alternative Name:	Interleukin-12 subunit α, CLMF, Cytotoxic lymphocyte maturation factor 35kDa subunit

Concentration:	1mg/ml

Purity Detail:	Protein G-affinity purified.

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Valy-1

Isotype:	Mouse IgG2b

Immunogen:	Recombinant human IL-12 (p35).

Source/Host:	Purified from concentrated hybridoma tissue culture supernatant.

Specificity:	Recognizes human IL-12 (p35) subunit present in IL-35 and IL-12.

Application:	ELISA Western blot (1:1‘000)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Interleukin-35 (IL-35) represents a member of the IL-12 cytokine family, which includes IL-12, IL-23 and IL-27. IL-35 is a heterodimer consisting of EBI3 (Epstein-Barr virus-induced gene 3) and IL-12 p35 [1-2]. Members of the IL-12 family such as IL-12, IL-23, IL-27 and IL-35 are cytokines that play a critical role in influencing T helper cell differentiation and inflammatory processes. IL-35 is highly expressed in mouse regulatory T cells (Treg) but not in effector or naive T cells. IL-35 induces proliferation of Treg cell populations but reduces activity of Th17 cell populations [2]. A study identifies IL-35 as a inhibitory cytokine required for the suppressive function of mouse Treg cells in vitro and in vivo [3].

General Literature References

IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expansion of regulatory T cells and suppression of Th17 cells: W. Niedbala, et al.; Eur. J. Immunol. 37, 3021 (2007), Abstract;

The inhibitory cytokine IL-35 contributes to regulatory T-cell function: L.W. Collison, et al.; Nature 450, 566 (2007), Abstract;

Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin 12 form a novel heterodimeric hematopoietin: O. Devergne, et al.; PNAS 94, 12041 (1997), Abstract; Full Text