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TIGAR (human) monoclonal antibody (Woody-1)

 
ALX-804-872-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:TP53-induced glycolysis and apoptosis regulator
 
Clone:Woody-1
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant human TIGAR (aa 1-270).
 
UniProt ID:Q9NQ88
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.
 
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Figure: Western blot analysis of human cell lines using MAb to TIGAR (human) (Woody-1) (Prod. No. ALX-804-872). Method: Total protein extracts from various human cell lines (293T,THP-1, U937, and HeLa) were run on SDS-PAGE and TIGAR was detected by MAb to TIGAR (human) (Woody-1) at 1:1’000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5’000 dilution for ECL detection.
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Product Literature References

Expression of TP53 isoforms p53β or p53γ enhances chemosensitivity in TP53(null) cell lines: E. Silden, et al.; PLoS One 8, e56276 (2013), Application(s): Western blot, Abstract; Full Text

General Literature References

TIGAR, a p53-inducible regulator of glycolysis and apoptosis: Bensaad, et al.; Cell 126, 107 (2006), Abstract;

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