ZBP1, mAb (Zippy-1)

[Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1, DNA-dependent activator of IFN-regulatory factors, DAI]



ALX-804-865-C100	100 µg	379.00 USD

Product Specification

Concentration:	0.5mg/ml

Purity Detail:	Protein G-affinity purified.

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Zippy-1

Isotype:	Mouse IgG2a

Immunogen:	Recombinant mouse ZBP1 (aa 1-411)

Source/Host:	Purified from concentrated hybridoma tissue culture supernatant.

Specificity:	Recognizes human and mouse ZBP1.

Application:	Immunoprecipitation (1:200) Western Blot (1:1’000)

Shipping:	SHIPPED ON BLUE ICE

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Figure: Detection of mouse ZBP1 / DAI in L929 cells by Western Blot using MAb to ZBP1 (Zippy-1) (Prod. No. AG-20B-0010).Method: Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3μg/ml (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to ZBP1 (Zippy-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. The multiple bands represent isoforms or degradation products of ZBP1 since they are down regulated upon ZBP1 siRNA treatment (data not shown).

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