MDA5 (human), mAb (Hely-1)

[Helicard, Melanoma differentiation-associated gene 5]



ALX-804-863-C100	100 µg	397.00 USD

Product Specification

Concentration:	1mg/ml

Purity Detail:	Protein G-affinity purified.

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Clone:	Hely-1

Isotype:	Mouse IgG1

Immunogen:	Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).

Source/Host:	Purified from concentrated hybridoma tissue cell culture supernatant.

Specificity:	Recognizes human MDA5.

Application:	ELISA Immunoprecipitation (1:100) Western Blot (1:1‘000)

Shipping:	SHIPPED ON BLUE ICE

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Miscellaneous/General:	MDA5 and RIG-I are highly conserved helicases involved in the innate immune response to virus. MDA-5 ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. After binding nonself-ligand, RIG-I and MDA5 interact via their CARD domains with the signaling-adaptor molecule, IPS-1 (also known as MAVS, VISA and Cardif), which leads to the induced expression of IFN-β, IRF3-target genes and NF- κB target genes to drive antiviral and inflammatory responses.

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties D.C. Kang, et al. PNAS 99 637 (2002) Abstract

Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses H. Kato, et al. Nature 441 101 (2006) Abstract