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Nesfatin-1 monoclonal antibody (Nesfaty-1)

 
ALX-804-854-C100 100 µg 385.00 USD
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Product Specification

Alternative Name:NEFA/nucleobindin2-encoded satiety- and fat-influencing protein, N-terminal fragment of NUCB2, Gastric cancer antigen Zg4
 
Clone:Nesfaty-1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant rat nesfatin-1 (aa 25-106).
 
UniProt ID:Q9JI85
 
Source:Purified from concentrated hybridoma tissue cell culture supernatant.
 
Species reactivity:Mouse, Rat
 
Specificity:Recognizes nesfatin-1 and precursor nucleobindin-2.
 
Applications:ELISA, IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake.
 

Product Literature References

Nesfatin-1 stimulates glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide secretion from STC-1 cells in vitro: N. Ramesh, et al.; Biochem. Biophys. Res. Commun. 462, 124 (2015), Application(s): Immunohistochemistry, Abstract;

General Literature References

Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature 443, 709 (2006), Abstract;

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