Cardif (human), mAb (Adri-1)
[CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, MAVS, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter]
| ALX-804-847-C100 | 100 µg | 397.00 USD |  |
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Product Specification
| Concentration: | 1mg/ml |
| Purity Detail: | Protein G-affinity purified. |
| Purity: | ≥95% (SDS-PAGE) |
| Formulation: | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Clone: | Adri-1 |
| Isotype: | Mouse IgG2b |
| Immunogen: | Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450). |
| Source/Host: | Purified from concentrated hybridoma tissue culture supernatant. |
| Specificity: | Recognizes human Cardif. |
| Application: | Immunocytochemistry (1:100) Immunohistochemistry (paraffin sections) Immunoprecipitation (1:200) Western Blot (1:1’000) |
| Shipping: | SHIPPED ON BLUE ICE |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
| Handling: | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Miscellaneous/General: | RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production. |
Figure: Cardif is cleaved in liver of a patient infected by the hepatitis C virus. Method: Protein extracts of liver biopsies from a patient with chronic hepatitis C (CHC) and as a control from a patient with alcoholic hepatitis (AH) were run on a SDS-gel and revealed with MAb to Cardif (Adri-1) (Prod. No. ALX-804-847). The arrows indicate endogenous human full-length or cleaved Cardif. Picture courtesy of Prof. Darius Moradpour, CHUV, Lausanne.
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Product Literature References
TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008), Abstract;
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus E. Meylan, et al. Nature 437 1167 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3 R.B. Seth, et al. Cell 122 669 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction T. Kawai, et al. Nat. Immunol. 6 981 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling L.G. Xu, et al. Mol. Cell 19 727 (2005) Abstract
CARD games between virus and host get a new player C.L. Johnson and M. Gale, Jr. Trends Immunol. 27 1 (2006) Abstract
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18-May-09 |
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