Product Details
| Clone: | Anto-1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human PIDD death domain (aa 776-910). |
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| UniProt ID: | Q9HB75 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Western blot analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1) (Prod. No.
ALX-804-837).
Method: Cell extracts (1x10
5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively."
Figure 2: Immunohistochemical staining of endogenous human PIDD in different human tissues (paraffin sections) using MAb to PIDD (Anto-1) (Prod. No.
ALX-804-837).
Method: Different human normal (A: Bronchus; B: Kidney; C: Lung) or cancer (D: Breast; E: Ovarian; F: Colo-rectal) tissues were stained with MAb to PIDD (Anto-1) by standard immunohistochemistry.
Pictures courtesy of Human Protein Atlas (www.proteinatlas.org)."
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Product Literature References
PIDD mediates the association of DNA-PKcs and ATR at stalled replication forks to facilitate the ATR signaling pathway: Y.F. Lin, et al.; Nucleic Acids Res.
46, 1847 (2018),
Application(s): Immunofluorescence,
Abstract;
Caspase-2 is involved in cell death induction by taxanes in breast cancer cells: M. Jelinek, et al.; Cancer Cell Int.
13, 42 (2013),
Application(s): Western blot,
Abstract;
Full Text
Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells to erlotinib/cell-cycle inhibitors combination treatment: M. Orzaez, et al.; Cell Death Dis.
3, e415 (2012),
Application(s): Western blot,
Abstract;
Full Text
Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway: A. Tinel, et al.; EMBO J.
26, 197 (2007),
Abstract;
General Literature References
PIDD mediates NF-kappaB activation in response to DNA damage: S. Janssens, et al.; Cell
123, 1079 (2005),
Abstract;
