CTRP7, mAb (Anna-1)



ALX-804-835-C100	100 µg	260.00 USD

Product Specification

Alternative Name:	Complement C1q tumor necrosis factor-related protein 7, C1qTNF7

Concentration:	1mg/ml

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Anna-1

Isotype:	Mouse IgG1

Immunogen:	Recombinant mouse CTRP7 (C1q tumor necrosis factor-α-related protein 7) (aa 18-290).

Source/Host:	Purified from concentrated hybridoma tissue culture supernatant.

Specificity:	Recognizes human and mouse CTRP7.

Application:	ELISA Immunohistochemistry (paraffin sections) Immunoprecipitation (1:200) Western Blot (1:1'000)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Adiponectin is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle. CTRP7 belongs to a highly conserved family of adiponectin paralogs designated as C1q tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. A member of this family, CTRP2 (mouse) has been reported to rapidly induce phosphorylation of AMP-activated protein kinase, acetyl-CoA carboxylase, and mitogen-activated protein kinase in C2C12 myotubes, which resulted in increased glycogen accumulation and fatty acid oxidation.

Figure 1: Western blot analysis of mouse CTRP7 using MAb to CTRP7 (Anna-1) (Prod. No. ALX-804-835). Method: Cell extracts (5x10⁵) from HEK 293T( lane1) or HEK293wt transfected with a plasmid coding for mouse CTRP7 (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to CTRP7 (Anna-1) (1:1’000) for 2 hours, followed by incubation with HRP-coupled anti-mouse secondary antibody (1:4’000).

Figure 2: Immunohistochemical staining of endogenous human CTRP7 in different human tissues (paraffin sections) using MAb to CTRP7 (Anna-1) (Prod. No. ALX-804-835). Method: Different human normal (A: Cerabral cortex; B: Colon; C: Kidney) or cancer (D: Prostate ; E: Colo-rectal; F: Pancreatic) tissues were stained with MAb to CTRP7 (Anna-1) by standard immunohistochemistry. Pictures courtesy of Human Protein Atlas (www.proteinatlas.org).

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