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PRUNE monoclonal antibody (4G3/4)

 
ALX-804-823-C050 50 µg 267.00 USD
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Product Specification

Clone:4G3/4
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Recombinant human PRUNE.
 
UniProt ID:Q86TP1
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human, Mouse
 
Applications:ICC, IHC (FS), WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:100-1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Not recommended for immunoprecipitation.
 
Formulation:Liquid. Affinity purified antibody in PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
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Figure: Detection of human PRUNE with MAb to PRUNE (4G3/4) (Prod. No. ALX-804-823).
Method: Control lysate from wild type 5YSY Neuroblastoma cells (20µg, lane 1), cell lysate from a stable clone 5YSY overexpressing  human PRUNE (20µg, lane 2) and purified human PRUNE protein from baculovirus expressing clone one passage purification (40ng, lane 3) were run on SDS-PAGE. PRUNE was detected by Western blot with ALX-804-823 at 1:500 dilution.
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Figure: Immunohistochemistry using MAb to PRUNE (4G3/4) (Prod. No. ALX-804-823) .
Method: Frozen section of mouse trachea stained with the MAb to PRUNE (4G3/4) at a 1:100 dilution.
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Figure: Immunohistochemistry using MAb to PRUNE (4G3/4) (Prod. No. ALX-804-823) .
Method: Frozen section of human cancerous breast stained with the MAb to PRUNE (4G3/4) at a 1:100 dilution.
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Product Literature References

Overexpression of h-prune in breast cancer is correlated with advanced disease status: M. Zollo, et al.; Clin. Cancer Res. 11, 199 (2005), Abstract;

General Literature References

Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis: A. D'Angelo, et al.; Cancer Cell 5, 137 (2004), Abstract;
Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity: A. Forus, et al.; Oncogene 20, 6881 (2001), Abstract;
Evidence for interaction between human PRUNE and nm23-H1 NDPKinase: A. Reymond, et al.; Oncogene 18, 7244 (1999), Abstract;

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