|Immunogen:||Recombinant human PRUNE.|
|Source:||Purified from concentrated hybridoma tissue culture supernatant.|
|Species reactivity:||Human, Mouse|
|Applications:||ICC, IHC (FS), WB|
|Recommended Dilutions/Conditions:||Immunocytochemistry (1:100)|
Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:100-1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
|Application Notes:||Not recommended for immunoprecipitation.|
|Formulation:||Liquid. Affinity purified antibody in PBS containing 0.02% sodium azide.|
|Use/Stability:||Stable for at least 1 year after receipt when stored at -20°C. |
|Handling:||Avoid freeze/thaw cycles.|
|Shipping:||Shipped on Blue Ice|
|Short Term Storage:||+4°C|
|Long Term Storage:||-20°C|
: Detection of human PRUNE with MAb to PRUNE (4G3/4) (Prod. No. ALX-804-823
: Control lysate from wild type 5YSY Neuroblastoma cells (20µg, lane 1), cell lysate from a stable clone 5YSY overexpressing human PRUNE (20µg, lane 2) and purified human PRUNE protein from baculovirus expressing clone one passage purification (40ng, lane 3) were run on SDS-PAGE. PRUNE was detected by Western blot with ALX-804-823
at 1:500 dilution.
: Immunohistochemistry using MAb to PRUNE (4G3/4) (Prod. No. ALX-804-823
Frozen section of mouse trachea stained with the MAb to PRUNE (4G3/4) at a 1:100 dilution.
Immunohistochemistry using MAb to PRUNE (4G3/4) (Prod. No. ALX-804-823
Frozen section of human cancerous breast stained with the MAb to PRUNE (4G3/4) at a 1:100 dilution.
Please mouse over
Product Literature References
Overexpression of h-prune in breast cancer is correlated with advanced disease status
: M. Zollo, et al.; Clin. Cancer Res. 11
, 199 (2005), Abstract
General Literature References
Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis
: A. D'Angelo, et al.; Cancer Cell 5
, 137 (2004), Abstract
Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity
: A. Forus, et al.; Oncogene 20
, 6881 (2001), Abstract
Evidence for interaction between human PRUNE and nm23-H1 NDPKinase
: A. Reymond, et al.; Oncogene 18
, 7244 (1999), Abstract