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Bcl-rambo (human) (CT) monoclonal antibody (Rocky-2)

 
ALX-804-821-C100 100 µg 313.00 USD
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Product Specification

Alternative Name:Bcl-2-like protein 13, Mil1 protein
 
Clone:Rocky-2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Bcl-rambo.
 
UniProt ID:Q9BXK5
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Specificity:Recognizes the C-terminus of human Bcl-rambo (aa 224-459).
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:300)
Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects endogenous protein by ICC, IP and WB.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-821
Figure: Immunofluorescence microscopy showing the detection of endogenous Bcl-rambo in HELA cells with the MAb to Bcl-rambo (human) (CT) (Rocky-2) (Prod. No. ALX-804-821).Method: Following fixation with 2% paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) (Prod. No. ALX-804-821) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection.
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804-821

General Literature References

Bcl-rambo beta, a special splicing variant with an insertion of an Alu-like cassette, promotes etoposide- and Taxol-induced cell death: P. Yi, et al.; FEBS Lett. 534, 61 (2003), Abstract;
Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension: T. Kataoka, et al.; J. Biol. Chem. 276, 19548 (2001), Abstract; Full Text

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