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NLRP3/NALP3 (human) monoclonal antibody (Nalpy3-b)

ALX-804-819-C100 100 µg 334.00 USD
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Product Specification

Alternative Name:NACHT-, LRR- and PYD-containing protein 3, CIAS1, Cryopyrin, Cold autoinflammatory syndrome 1 protein, PYPAF1, PYRIN-containing APAF1-like protein 1
Immunogen:Recombinant human NLRP3/NALP3 (NACHT-, LRR- and PYD-containing protein 3) (pyrin domain).
UniProt ID:Q96P20
Source:Purified from concentrated hybridoma tissue culture supernatant.
Species reactivity:Human
Specificity:Detects endogenous protein by IP and WB.
Applications:IHC (FS), IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (1:50)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Figure: Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b) (Prod. No. ALX-804-819). Method: 5µM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b). Picture courtesy of Dr. Alain Kummer, University Medical Center Utrecht, The Netherlands.
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Product Literature References

Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early-onset autoinflammatory disease: Q. Zhou, et al.; Nat. Genet. 48, 67 (2016), Application(s): Immunoblots of total cell lysates, Abstract;
Dysregulated production of interleukin-1β upon activation of the NLRP3 inflammasome in patients with familial Mediterranean fever: A. Repa, et al.; Hum. Immunol. 76, 488 (2015), Application(s): Western Blot, Abstract;
Mechanism of hepatic inflammation during hepatitis C virus infection: A.A. Negash, et al.; (2013), (Thesis paper), Application(s): WB, IHC of human liver cells, Abstract;
NLRP3 inflammasome activation in retinal pigment epithelial cells by lysosomal destabilization: implications for age-related macular degeneration: W.A. Tseng, et al.; Invest. Ophthalmol. Vis. Sci. 54, 110 (2013), Application(s): Immunohistochemistry of paraffin-embedded tissue, Abstract; Full Text
Staphylococcus aureus Activates the NLRP3 Inflammasome in Human and Rat Conjunctival Goblet Cells: V. McGilligan, et al.; PLoS One 8, e74010 (2013), Application(s): WB, IHC, and Cell culture of rat and human cells, Abstract; Full Text
Complement Protein C1q Directs Macrophage Polarization and Limits Inflammasome Activity during the Uptake of Apoptotic cells: M. Benoit, et al.; J. Immunol. 188, 5682 (2012), Application(s): WB, Cell culture of human lymphocytes and macrophages, Abstract; Full Text
Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases: M.T. Shio, et al.; PLoS Pathog. 5, e1000559 (2009), Abstract;
Polymorphism in a gene coding for the inflammasome component NALP3 and recurrent vulvovaginal candidiasis in women with vulvar vestibulitis syndrome: A. Lev-Sagie, et al.; Am. J. Obstet. Gynecol. 200, 303 (2009), Application(s): Immunohistochemistry of paraffin-embedded tissue, Abstract;
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response: J.A. Kummer, et al.; J. Histochem. Cytochem. 55, 443 (2007), Abstract;
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004), Abstract;

General Literature References

Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon and J. Tschopp; Cell 117, 561 (2004), Abstract;
Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties: W. O'Connor, Jr., et al.; J. Immunol. 171, 6329 (2003), Abstract;
Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation: M. Chamaillard, et al.; Cell. Microbiol. 5, 581 (2003), Abstract; Full Text

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