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NLRP3/NALP3 (human) monoclonal antibody (Nalpy3-a)

ALX-804-818-C100 100 µg 334.00 USD

Product Specification

Alternative Name:CIAS1, NACHT-, LRR- and PYD-containing protein 3, Cryopyrin, Cold autoinflammatory syndrome 1 protein, PYPAF1, PYRIN-containing APAF1-like protein 1
Immunogen:Recombinant human NLRP3/NALP3 (NACHT-, LRR- and PYD-containing protein 3) (pyrin domain).
UniProt ID:Q96P20
Source:Purified from concentrated hybridoma tissue culture supernatant.
Species reactivity:Human
Specificity:Detects endogenous protein by ICC and WB.
Applications:ICC, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Figure: Immunofluorescence microscopy showing the detection of endogenous NLRP3/NALP3 in THP1 cells using NLRP3/NALP3 (human), mAb (Nalpy3-a) (Prod. No. ALX-804-818).Method: Following fixation with paraformaldehyde 3.7%, THP1 cells were incubated with NLRP3/NALP3 (human), mAb (Nalpy3-a) (Prod. No. ALX-804-818) at a 1:100 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection.
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Product Literature References

NLRP3 tyrosine phosphorylation is controlled by protein tyrosine phosphatase PTPN22: M. R. Spalinger, et al.; J. Clin. Invest. 126, 1783 (2016), Application(s): Western blotting and immunoprecipitation, Abstract; Full Text
NLRP3 deficiency improves angiotensin II-induced hypertension but not fetal growth restriction during pregnancy: K. Shirasuna, et al.; Endocrinology 156, 4281 (2015), Application(s): Western Blot, Abstract; Full Text
Reconstituted AIM2 inflammasome in cell-free system: N. Kaneko, et al. ; J. Immunol. Methods 426, 76 (2015), Abstract;
Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases: M.T. Shio, et al.; PLoS Pathog. 5, e1000559 (2009), Abstract;
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004), Abstract;

General Literature References

Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon and J. Tschopp; Cell 117, 561 (2004), Abstract;
Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties: W. O'Connor, Jr., et al.; J. Immunol. 171, 6329 (2003), Abstract;
Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation: M. Chamaillard, et al.; Cell. Microbiol. 5, 581 (2003), Abstract; Full Text

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