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BAP31 monoclonal antibody (CC-1)

 
ALX-804-812-C100 100 µg 280.00 USD
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Product Specification

Alternative Name:DXS1357E, B cell receptor-associated protein BAP31, BCR-associated protein BAP31, p28 BAP31, CDM protein, 6C6-AG tumor-associated antigen, BCAP31
 
Clone:CC-1
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Solubilized membranes of the IMR-32 human neuroblastoma cell line.
 
UniProt ID:P51572
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
Bovine, Hamster, Primate
 
Specificity:Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31.
 
Crossreactivity:Does not recognize mouse and rat BAP31.
 
Applications:ELISA, ICC, IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:ELISA (capture)
Immunocytochemistry (1:1,000-1:10,000)
Immunohistochemistry (frozen sections, 1:1,000-1:10,000)
Western Blot (1:1,000-1:6,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Works on endogenous in ICC, IHC and WB.
 
Formulation:Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca2+-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2.
 

Product Literature References

Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules: J.J. Ladasky, et al.; J. Immunol. 177, 6172 (2006), Abstract;
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils: K. Zen, et al.; J. Biol. Chem. 279, 44924 (2004), Abstract; Full Text
Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells: H.A. Manley and V.A. Lennon; J. Histochem. Cytochem. 49, 1235 (2001), Abstract; Full Text

General Literature References

Bap31 Is an Itinerant Protein that Moves between the Peripheral ER and a Juxtanuclear Compartment Related to ER-associated Degradation: Y. Wakana, et al.; Mol. Biol. Cell 19, 1825 (2008), Abstract; Full Text
Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol: D.G. Breckenridge, et al.; J. Cell. Biol. 160, 1115 (2003), Abstract; Full Text
Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol. 20, 6731 (2000), Abstract; Full Text

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