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LAG-3 (human) monoclonal antibody (17B4) (ATTO 488 conjugate)

ALX-804-806TD-T100 100 tests ATTO 488 516.00 USD
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Product Specification

Alternative Name:Lymphocyte activation gene-3, FDC protein, CD223
Immunogen:Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
UniProt ID:P18627
Species reactivity:Human
Specificity:Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3.
Applications:Flow Cytometry, ICC
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified from concentrated hybridoma tissue culture supernatant by Protein A Sepharose™ CL-4B Flow Fast Column.
Formulation:Liquid. In PBS containing 0.1M sodium bicarbonate, 50mM Tris, 1% BSA and 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored as recommended.
Handling:Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:+4°C
Scientific Background:The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.
Technical Info/Product Notes:1 test means: 1µl of MAb is used to stain 500'000 cells in a sample volume of 50µl.

ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000).

SEPHAROSE is a trademark of GE Healthcare companies.
Figure: Detection of endogenous human LAG-3 by FACS analysis using LAG-3 (human), mAb (17B4) (ATTO 488) (Prod. No. ALX-804-806TD). Method: Human PBMC were stimulated (B) or not (A) with 1µg/ml of superantigen SEB. After 2 days, PBMC were stained with 10µg/ml (1µg/0.5x106 cells) of LAG-3 (human), mAb (17B4) (ATTO 488) (Prod. No. ALX-804-806TD) and analyzed by flow cytometry.
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Product Literature References

Identification of tonsillar CD4+CD25-LAG3+ T cells as naturally occurring IL-10-producing regulatory T cells in human lymphoid tissue: S. Sumitomo, et al.; J. Autoimmun. 76, 75 (2017), Application(s): Flow cytometry and immunofluorescence, Abstract;
Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation: C. Casati, et al.; J. Immunol. 180, 3782 (2008), Abstract;
A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006), Abstract;
Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway: E.D. Carlo, et al.; J. Pathol. 205, 82 (2005), Abstract;
The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells: L. Macon-Lemaitre and F. Triebel; Immunology 115, 170 (2005), Abstract;
T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts: C.E. Demeure, et al.; Eur. J. Cancer 37, 1709 (2001), Abstract;
CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling: S. Hannier, et al.; J. Immunol. 161, 4058 (1998), Abstract; Full Text
Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS 94, 5744 (1997), Abstract; Full Text
Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics 39, 213 (1994), Abstract;
Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens: E. Baixeras, et al.; J. Exp. Med. 176, 327 (1992), Abstract; Full Text

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